Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis-gas chromatography

Hiroaki Yokoi, Yasuyuki Ishida, Hajime Ohtani, Shin Tsuge, Tetsuya Sonoda, Toshihiro Ona

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Pyrolysis-gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer was applied to the precise determination of the 'within-tree variation' of the ratio of syringyl and guaiacyl units (S/G ratio) for lignin in Eucalyptus camaldulensis. On the pyrogram of the Eucalyptus obtained at 450°C, many characteristic peaks derived from syringyl and guaiacyl units of lignin were reproducibly observed, together with those from cellulose and hemicellulose. On the basis of the relative intensities of those characteristic peaks relating to the syringyl and guaiacyl units, the 'within-tree variation' of the S/G ratio was determined precisely in about 1 h using only about 0.1 mg of the powdered wood sample with a 2% relative standard deviation. The results obtained were also compared with those obtained by the thioacidolysis method.

Original languageEnglish
Pages (from-to)669-674
Number of pages6
JournalAnalyst
Volume124
Issue number5
DOIs
Publication statusPublished - Jun 7 1999
Externally publishedYes

Fingerprint

Eucalyptus
Lignin
Gas chromatography
Gas Chromatography
pyrolysis
lignin
gas chromatography
Pyrolysis
Cellulose
cellulose
Wood
hemicellulose
method

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis-gas chromatography. / Yokoi, Hiroaki; Ishida, Yasuyuki; Ohtani, Hajime; Tsuge, Shin; Sonoda, Tetsuya; Ona, Toshihiro.

In: Analyst, Vol. 124, No. 5, 07.06.1999, p. 669-674.

Research output: Contribution to journalArticle

Yokoi, Hiroaki ; Ishida, Yasuyuki ; Ohtani, Hajime ; Tsuge, Shin ; Sonoda, Tetsuya ; Ona, Toshihiro. / Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis-gas chromatography. In: Analyst. 1999 ; Vol. 124, No. 5. pp. 669-674.
@article{532351fdeb9345b38bf970ae0ea47e96,
title = "Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis-gas chromatography",
abstract = "Pyrolysis-gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer was applied to the precise determination of the 'within-tree variation' of the ratio of syringyl and guaiacyl units (S/G ratio) for lignin in Eucalyptus camaldulensis. On the pyrogram of the Eucalyptus obtained at 450°C, many characteristic peaks derived from syringyl and guaiacyl units of lignin were reproducibly observed, together with those from cellulose and hemicellulose. On the basis of the relative intensities of those characteristic peaks relating to the syringyl and guaiacyl units, the 'within-tree variation' of the S/G ratio was determined precisely in about 1 h using only about 0.1 mg of the powdered wood sample with a 2{\%} relative standard deviation. The results obtained were also compared with those obtained by the thioacidolysis method.",
author = "Hiroaki Yokoi and Yasuyuki Ishida and Hajime Ohtani and Shin Tsuge and Tetsuya Sonoda and Toshihiro Ona",
year = "1999",
month = "6",
day = "7",
doi = "10.1039/a809217f",
language = "English",
volume = "124",
pages = "669--674",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "5",

}

TY - JOUR

T1 - Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis-gas chromatography

AU - Yokoi, Hiroaki

AU - Ishida, Yasuyuki

AU - Ohtani, Hajime

AU - Tsuge, Shin

AU - Sonoda, Tetsuya

AU - Ona, Toshihiro

PY - 1999/6/7

Y1 - 1999/6/7

N2 - Pyrolysis-gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer was applied to the precise determination of the 'within-tree variation' of the ratio of syringyl and guaiacyl units (S/G ratio) for lignin in Eucalyptus camaldulensis. On the pyrogram of the Eucalyptus obtained at 450°C, many characteristic peaks derived from syringyl and guaiacyl units of lignin were reproducibly observed, together with those from cellulose and hemicellulose. On the basis of the relative intensities of those characteristic peaks relating to the syringyl and guaiacyl units, the 'within-tree variation' of the S/G ratio was determined precisely in about 1 h using only about 0.1 mg of the powdered wood sample with a 2% relative standard deviation. The results obtained were also compared with those obtained by the thioacidolysis method.

AB - Pyrolysis-gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer was applied to the precise determination of the 'within-tree variation' of the ratio of syringyl and guaiacyl units (S/G ratio) for lignin in Eucalyptus camaldulensis. On the pyrogram of the Eucalyptus obtained at 450°C, many characteristic peaks derived from syringyl and guaiacyl units of lignin were reproducibly observed, together with those from cellulose and hemicellulose. On the basis of the relative intensities of those characteristic peaks relating to the syringyl and guaiacyl units, the 'within-tree variation' of the S/G ratio was determined precisely in about 1 h using only about 0.1 mg of the powdered wood sample with a 2% relative standard deviation. The results obtained were also compared with those obtained by the thioacidolysis method.

UR - http://www.scopus.com/inward/record.url?scp=0033061075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033061075&partnerID=8YFLogxK

U2 - 10.1039/a809217f

DO - 10.1039/a809217f

M3 - Article

AN - SCOPUS:0033061075

VL - 124

SP - 669

EP - 674

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 5

ER -