Chd2 interacts with H3.3 to determine myogenic cell fate

Akihito Harada, Seiji Okada, Daijiro Konno, Jun Odawara, Tomohiko Yoshimi, Saori Yoshimura, Hiromi Kumamaru, Hirokazu Saiwai, Toshiaki Tsubota, Hitoshi Kurumizaka, Koichi Akashi, Taro Tachibana, Anthony N. Imbalzano, Yasuyuki Ohkawa

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.

Original languageEnglish
Pages (from-to)2994-3007
Number of pages14
JournalEMBO Journal
Volume31
Issue number13
DOIs
Publication statusPublished - Jul 4 2012

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DNA Helicases
Genes
Cell Differentiation
Chromatin Assembly and Disassembly
Essential Genes
Regulator Genes
Histones
Transcriptional Activation
Transcription Factors
Gene expression
Genome
Chromatin
Gene Expression
Chemical activation
Enzymes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Chd2 interacts with H3.3 to determine myogenic cell fate. / Harada, Akihito; Okada, Seiji; Konno, Daijiro; Odawara, Jun; Yoshimi, Tomohiko; Yoshimura, Saori; Kumamaru, Hiromi; Saiwai, Hirokazu; Tsubota, Toshiaki; Kurumizaka, Hitoshi; Akashi, Koichi; Tachibana, Taro; Imbalzano, Anthony N.; Ohkawa, Yasuyuki.

In: EMBO Journal, Vol. 31, No. 13, 04.07.2012, p. 2994-3007.

Research output: Contribution to journalArticle

Harada, A, Okada, S, Konno, D, Odawara, J, Yoshimi, T, Yoshimura, S, Kumamaru, H, Saiwai, H, Tsubota, T, Kurumizaka, H, Akashi, K, Tachibana, T, Imbalzano, AN & Ohkawa, Y 2012, 'Chd2 interacts with H3.3 to determine myogenic cell fate', EMBO Journal, vol. 31, no. 13, pp. 2994-3007. https://doi.org/10.1038/emboj.2012.136
Harada, Akihito ; Okada, Seiji ; Konno, Daijiro ; Odawara, Jun ; Yoshimi, Tomohiko ; Yoshimura, Saori ; Kumamaru, Hiromi ; Saiwai, Hirokazu ; Tsubota, Toshiaki ; Kurumizaka, Hitoshi ; Akashi, Koichi ; Tachibana, Taro ; Imbalzano, Anthony N. ; Ohkawa, Yasuyuki. / Chd2 interacts with H3.3 to determine myogenic cell fate. In: EMBO Journal. 2012 ; Vol. 31, No. 13. pp. 2994-3007.
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