Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

Masayasu Hayashi, Kazumitsu Maehara, Akihito Harada, Yuichiro Semba, Kensuke Kudo, Hidehisa Takahashi, Shinya Oki, Chikara Meno, Kenji Ichiyanagi, Koichi Akashi, Yasuyuki Ohkawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.

Original languageEnglish
Pages (from-to)780-792
Number of pages13
JournalJournal of Cellular Biochemistry
Volume117
Issue number3
DOIs
Publication statusPublished - Mar 2016

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Histone Code
Endogenous Retroviruses
Stem cells
Histones
Clustered Regularly Interspaced Short Palindromic Repeats
Retroelements
Spermatogenesis
Chromatin
Tumors
Neoplasms
Mouse Embryonic Stem Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2. / Hayashi, Masayasu; Maehara, Kazumitsu; Harada, Akihito; Semba, Yuichiro; Kudo, Kensuke; Takahashi, Hidehisa; Oki, Shinya; Meno, Chikara; Ichiyanagi, Kenji; Akashi, Koichi; Ohkawa, Yasuyuki.

In: Journal of Cellular Biochemistry, Vol. 117, No. 3, 03.2016, p. 780-792.

Research output: Contribution to journalArticle

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abstract = "Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.",
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AU - Hayashi, Masayasu

AU - Maehara, Kazumitsu

AU - Harada, Akihito

AU - Semba, Yuichiro

AU - Kudo, Kensuke

AU - Takahashi, Hidehisa

AU - Oki, Shinya

AU - Meno, Chikara

AU - Ichiyanagi, Kenji

AU - Akashi, Koichi

AU - Ohkawa, Yasuyuki

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AB - Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.

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