CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer

Genta Sawada, Hiroki Ueo, Tae Matsumura, Ryutaro Uchi, Masahisa Ishibashi, Kosuke Mima, Junji Kurashige, Yusuke Takahashi, Sayori Akiyoshi, Tomoya Sudo, Keishi Sugimachi, Yuichiro Doki, Masaki Mori, Koshi Mimori

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The chromodomain helicase DNA-binding (CHD) family comprises a class of chromatin remodeling enzymes. Previous studies suggest that CHD8 may negatively regulate various genes and signaling pathways, such as the Wnt/β-catenin pathway. However, few studies have investigated the role of CHD8 in cancer cells. We analyzed the expression of CHD8 in cancer lesions and corresponding non-cancerous tissues to demonstrate the prognostic significance of CHD8 expression in 101 cases of gastric cancer. We also investigated the functional implications of aberrant CHD8 expression by conducting gene set enrichment analysis (GSEA). Expression of CHD8 mRNA was significantly lower in gastric cancer tissues compared to that in corresponding normal tissues (P=0.003). In multivariate analysis for overall survival, we found that CHD8 expression was an independent prognostic factor in gastric cancer. Moreover, GSEA revealed that CHD8 was significantly associated with genes involved in the Wnt/β-catenin pathway and in the cell cycle. In addition, knockdown of CHD8 expression in the gastric cancer cell lines, MKN45 and NUGC4, promoted proliferation. In conclusion, the present study suggests that loss of CHD8 expression may be a novel indicator for biological aggressiveness in gastric cancer.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalOncology reports
Volume30
Issue number3
DOIs
Publication statusPublished - Sep 1 2013

Fingerprint

Catenins
Stomach Neoplasms
Cell Cycle
Wnt Signaling Pathway
Genes
DNA Helicases
Chromatin Assembly and Disassembly
Neoplasms
Multivariate Analysis
Gene Expression
Cell Line
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer. / Sawada, Genta; Ueo, Hiroki; Matsumura, Tae; Uchi, Ryutaro; Ishibashi, Masahisa; Mima, Kosuke; Kurashige, Junji; Takahashi, Yusuke; Akiyoshi, Sayori; Sudo, Tomoya; Sugimachi, Keishi; Doki, Yuichiro; Mori, Masaki; Mimori, Koshi.

In: Oncology reports, Vol. 30, No. 3, 01.09.2013, p. 1137-1142.

Research output: Contribution to journalArticle

Sawada, G, Ueo, H, Matsumura, T, Uchi, R, Ishibashi, M, Mima, K, Kurashige, J, Takahashi, Y, Akiyoshi, S, Sudo, T, Sugimachi, K, Doki, Y, Mori, M & Mimori, K 2013, 'CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer', Oncology reports, vol. 30, no. 3, pp. 1137-1142. https://doi.org/10.3892/or.2013.2597
Sawada, Genta ; Ueo, Hiroki ; Matsumura, Tae ; Uchi, Ryutaro ; Ishibashi, Masahisa ; Mima, Kosuke ; Kurashige, Junji ; Takahashi, Yusuke ; Akiyoshi, Sayori ; Sudo, Tomoya ; Sugimachi, Keishi ; Doki, Yuichiro ; Mori, Masaki ; Mimori, Koshi. / CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer. In: Oncology reports. 2013 ; Vol. 30, No. 3. pp. 1137-1142.
@article{04d06e5021c54d8da0b2b37be37649a7,
title = "CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer",
abstract = "The chromodomain helicase DNA-binding (CHD) family comprises a class of chromatin remodeling enzymes. Previous studies suggest that CHD8 may negatively regulate various genes and signaling pathways, such as the Wnt/β-catenin pathway. However, few studies have investigated the role of CHD8 in cancer cells. We analyzed the expression of CHD8 in cancer lesions and corresponding non-cancerous tissues to demonstrate the prognostic significance of CHD8 expression in 101 cases of gastric cancer. We also investigated the functional implications of aberrant CHD8 expression by conducting gene set enrichment analysis (GSEA). Expression of CHD8 mRNA was significantly lower in gastric cancer tissues compared to that in corresponding normal tissues (P=0.003). In multivariate analysis for overall survival, we found that CHD8 expression was an independent prognostic factor in gastric cancer. Moreover, GSEA revealed that CHD8 was significantly associated with genes involved in the Wnt/β-catenin pathway and in the cell cycle. In addition, knockdown of CHD8 expression in the gastric cancer cell lines, MKN45 and NUGC4, promoted proliferation. In conclusion, the present study suggests that loss of CHD8 expression may be a novel indicator for biological aggressiveness in gastric cancer.",
author = "Genta Sawada and Hiroki Ueo and Tae Matsumura and Ryutaro Uchi and Masahisa Ishibashi and Kosuke Mima and Junji Kurashige and Yusuke Takahashi and Sayori Akiyoshi and Tomoya Sudo and Keishi Sugimachi and Yuichiro Doki and Masaki Mori and Koshi Mimori",
year = "2013",
month = "9",
day = "1",
doi = "10.3892/or.2013.2597",
language = "English",
volume = "30",
pages = "1137--1142",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "3",

}

TY - JOUR

T1 - CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer

AU - Sawada, Genta

AU - Ueo, Hiroki

AU - Matsumura, Tae

AU - Uchi, Ryutaro

AU - Ishibashi, Masahisa

AU - Mima, Kosuke

AU - Kurashige, Junji

AU - Takahashi, Yusuke

AU - Akiyoshi, Sayori

AU - Sudo, Tomoya

AU - Sugimachi, Keishi

AU - Doki, Yuichiro

AU - Mori, Masaki

AU - Mimori, Koshi

PY - 2013/9/1

Y1 - 2013/9/1

N2 - The chromodomain helicase DNA-binding (CHD) family comprises a class of chromatin remodeling enzymes. Previous studies suggest that CHD8 may negatively regulate various genes and signaling pathways, such as the Wnt/β-catenin pathway. However, few studies have investigated the role of CHD8 in cancer cells. We analyzed the expression of CHD8 in cancer lesions and corresponding non-cancerous tissues to demonstrate the prognostic significance of CHD8 expression in 101 cases of gastric cancer. We also investigated the functional implications of aberrant CHD8 expression by conducting gene set enrichment analysis (GSEA). Expression of CHD8 mRNA was significantly lower in gastric cancer tissues compared to that in corresponding normal tissues (P=0.003). In multivariate analysis for overall survival, we found that CHD8 expression was an independent prognostic factor in gastric cancer. Moreover, GSEA revealed that CHD8 was significantly associated with genes involved in the Wnt/β-catenin pathway and in the cell cycle. In addition, knockdown of CHD8 expression in the gastric cancer cell lines, MKN45 and NUGC4, promoted proliferation. In conclusion, the present study suggests that loss of CHD8 expression may be a novel indicator for biological aggressiveness in gastric cancer.

AB - The chromodomain helicase DNA-binding (CHD) family comprises a class of chromatin remodeling enzymes. Previous studies suggest that CHD8 may negatively regulate various genes and signaling pathways, such as the Wnt/β-catenin pathway. However, few studies have investigated the role of CHD8 in cancer cells. We analyzed the expression of CHD8 in cancer lesions and corresponding non-cancerous tissues to demonstrate the prognostic significance of CHD8 expression in 101 cases of gastric cancer. We also investigated the functional implications of aberrant CHD8 expression by conducting gene set enrichment analysis (GSEA). Expression of CHD8 mRNA was significantly lower in gastric cancer tissues compared to that in corresponding normal tissues (P=0.003). In multivariate analysis for overall survival, we found that CHD8 expression was an independent prognostic factor in gastric cancer. Moreover, GSEA revealed that CHD8 was significantly associated with genes involved in the Wnt/β-catenin pathway and in the cell cycle. In addition, knockdown of CHD8 expression in the gastric cancer cell lines, MKN45 and NUGC4, promoted proliferation. In conclusion, the present study suggests that loss of CHD8 expression may be a novel indicator for biological aggressiveness in gastric cancer.

UR - http://www.scopus.com/inward/record.url?scp=84880641966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880641966&partnerID=8YFLogxK

U2 - 10.3892/or.2013.2597

DO - 10.3892/or.2013.2597

M3 - Article

C2 - 23835524

AN - SCOPUS:84880641966

VL - 30

SP - 1137

EP - 1142

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 3

ER -