TY - JOUR
T1 - Chemical and biological roles of zinc in a porous titanium dioxide layer formed by micro-arc oxidation
AU - Shimabukuro, Masaya
AU - Tsutsumi, Yusuke
AU - Nozaki, Kosuke
AU - Chen, Peng
AU - Yamada, Risa
AU - Ashida, Maki
AU - Doi, Hisashi
AU - Nagai, Akiko
AU - Hanawa, Takao
N1 - Funding Information:
Acknowledgments: This study was supported by the Research Center for Biomedical Engineering, Tokyo Medical and Dental University, Project “Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development” and project “Cooperative project amount medicine, dentistry, and engineering for medical innovation-Construction of creative scientific research of the viable material via integration of biology and engineering” by the Ministry of Education, Culture, Sports, Science and Engineering, Japan.
Publisher Copyright:
© 2019 by the authors.
PY - 2019
Y1 - 2019
N2 - This study investigated the time transient effect of zinc (Zn) in the porous titanium dioxide formed by micro-arc oxidation (MAO) treatment routinely performed for Zn-containing electrolytes. The aim of our analysis was to understand the changes in both the chemical and biological properties of Zn in physiological saline. The morphology of the Zn-incorporated MAO surface did not change, and a small amount of Zn ions were released at early stages of incubation in saline. We observed a decrease in Zn concentration in the oxide layer because its release and chemical state (Zn2+ compound to ZnO) changed over time during incubation in saline. In addition, the antibacterial property of the Zn-incorporated MAO surface developed at late periods after the incubation process over a course of 28 days. Furthermore, osteogenic cells were able to proliferate and were calcified on the specimens with Zn. The changes related to Zn in saline had non-toxic effects on the osteogenic cells. In conclusion, the time transient effect of Zn in a porous titanium dioxide layer was beneficial to realize dual functions, namely the antibacterial property and osteogenic cell compatibility. Our study suggests the importance of the chemical state changes of Zn to control its chemical and biological properties.
AB - This study investigated the time transient effect of zinc (Zn) in the porous titanium dioxide formed by micro-arc oxidation (MAO) treatment routinely performed for Zn-containing electrolytes. The aim of our analysis was to understand the changes in both the chemical and biological properties of Zn in physiological saline. The morphology of the Zn-incorporated MAO surface did not change, and a small amount of Zn ions were released at early stages of incubation in saline. We observed a decrease in Zn concentration in the oxide layer because its release and chemical state (Zn2+ compound to ZnO) changed over time during incubation in saline. In addition, the antibacterial property of the Zn-incorporated MAO surface developed at late periods after the incubation process over a course of 28 days. Furthermore, osteogenic cells were able to proliferate and were calcified on the specimens with Zn. The changes related to Zn in saline had non-toxic effects on the osteogenic cells. In conclusion, the time transient effect of Zn in a porous titanium dioxide layer was beneficial to realize dual functions, namely the antibacterial property and osteogenic cell compatibility. Our study suggests the importance of the chemical state changes of Zn to control its chemical and biological properties.
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U2 - 10.3390/coatings9110705
DO - 10.3390/coatings9110705
M3 - Article
AN - SCOPUS:85075581398
SN - 2079-6412
VL - 9
JO - Coatings
JF - Coatings
IS - 11
M1 - 705
ER -