Chemical Modification of Molecularly Smooth Mica Surface and Protein Attachment

Hiroshi Okusa, Kazue Kurihara, Toyoki Kunitake

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Activation and chemical modification of the molecularly smooth mica surface were investigated. Exposure to water vapor plasma made the mica surface reactive enough to be silanized with gaseous chlorosilanes or alkoxysilanes. Conditions for silanization were optimized to immobilize various functional groups onto mica surface. By selection of appropriate reaction conditions, functional units such as trifluoromethyl, mercapto, amino, dimethylamino, isocyanate, urea, 4,5-dihydroimidazole, and chlorobenzyl groups were covalently bound to the mica surface at densities close to monolayer coverage. Improved simulation of X-ray photoelectron spectral data was essential for the surface characterization. Urease could be immobilized on a chlorobenzylated mica surface. Immobilized urease was retained upon washing with an aqueous surfactant and gave atomic force microscopy (AFM) images of 10 × 20–30 nm size which appeared to be enlarged due to the finite size of the AFM tip. These images were stable during several repeated scans.

Original languageEnglish
Pages (from-to)3577-3581
Number of pages5
JournalLangmuir
Volume10
Issue number10
DOIs
Publication statusPublished - Oct 1 1994

Fingerprint

Mica
Chemical modification
mica
attachment
Membrane Proteins
proteins
Proteins
Urease
Atomic force microscopy
atomic force microscopy
chlorosilanes
Isocyanates
isocyanates
washing
Steam
Photoelectrons
ureas
Washing
Surface-Active Agents
Urea

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Chemical Modification of Molecularly Smooth Mica Surface and Protein Attachment. / Okusa, Hiroshi; Kurihara, Kazue; Kunitake, Toyoki.

In: Langmuir, Vol. 10, No. 10, 01.10.1994, p. 3577-3581.

Research output: Contribution to journalArticle

Okusa, Hiroshi ; Kurihara, Kazue ; Kunitake, Toyoki. / Chemical Modification of Molecularly Smooth Mica Surface and Protein Attachment. In: Langmuir. 1994 ; Vol. 10, No. 10. pp. 3577-3581.
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