Chemical stress-responsive genes from the lignin-degrading fungus Phanerochaete chrysosporium exposed to dibenzo-p-dioxin

Hiroyuki Kurihara, Hiroyuki Wariishi, Hiroo Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The stress-responsive genes expressed against the exogenous addition of dibenzo-p-dioxin from the lignin-degrading basidiomycete, Phanerochaete chrysosporium, were determined utilizing a differential display reverse transcription-PCR technique. Six cDNA fragments, exhibiting a high homology with various proteins from other microorganisms, were identified via a BLAST search; that is, NADH-ubiquinone oxidoreductase (NUO), ATP/ADP carrier, uric acid-xanthine permease, manganese superoxide dismutase, 3-hydroxybutyryl-coenzyme A dehydrogenase, and cytoskeletal protein. The expression of NUO was also up-regulated by catechol and trihydroxybenzene but not by dibenzofuran, suggesting that NUO expression was initiated by the formation of quinone products through the reaction of extracellular one-electron oxidizing enzymes with dibenzo-p-dioxin.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalFEMS microbiology letters
Volume212
Issue number2
DOIs
Publication statusPublished - Jul 2 2002

Fingerprint

Phanerochaete
Electron Transport Complex I
Lignin
Fungi
ATP Translocases Mitochondrial ADP
Genes
Basidiomycota
Xanthine
Cytoskeletal Proteins
Membrane Transport Proteins
Uric Acid
Reverse Transcription
Superoxide Dismutase
Oxidoreductases
Complementary DNA
Electrons
Polymerase Chain Reaction
Enzymes
dibenzo(1,4)dioxin
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Chemical stress-responsive genes from the lignin-degrading fungus Phanerochaete chrysosporium exposed to dibenzo-p-dioxin. / Kurihara, Hiroyuki; Wariishi, Hiroyuki; Tanaka, Hiroo.

In: FEMS microbiology letters, Vol. 212, No. 2, 02.07.2002, p. 217-220.

Research output: Contribution to journalArticle

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