Chemical tools for targeted mutagenesis of DNA based on triple helix formation

Fumi Nagatsugi, Shigeki Sasaki

Research output: Contribution to journalReview article

10 Citations (Scopus)

Abstract

The development of methods for targeted mutagenesis shows promise as an alternative form of gene therapy. Triple helix-forming oligonucleotides (TFOs) provide an attractive strategy for inducing mutations. Especially, alkylation of nucleobases with functionalized TFOs would have potential for site-directed mutation. Several studies have demonstrated that treatment of mammalian cells with TFOs can be exploited to introduce desired sequence changes and point mutations. This review summarizes targeted mutagenesis using reactive TFOs, including studies with photo reactive psolaren derivatives as well as a new reactive derivative recently developed by our group.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 1 2004

Fingerprint

Oligonucleotides
Mutagenesis
DNA
Mutation
Alkylation
Point Mutation
Genetic Therapy
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Chemical tools for targeted mutagenesis of DNA based on triple helix formation. / Nagatsugi, Fumi; Sasaki, Shigeki.

In: Biological and Pharmaceutical Bulletin, Vol. 27, No. 4, 01.04.2004, p. 463-467.

Research output: Contribution to journalReview article

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