Abstract
A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.
| Original language | English |
|---|---|
| Pages (from-to) | 2027-2043 |
| Number of pages | 17 |
| Journal | Pure and Applied Chemistry |
| Volume | 84 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 25 2012 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
Cite this
Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform. / Guo, Shuai; Nakano, Koji; Nakajima, Hizuru; Uchiyama, Katsumi; Hemmi, Akihide; Yamasaki, Yoshikazu; Morooka, Shigeharu; Ishimatsu, Ryoichi; Imato, Toshihiko.
In: Pure and Applied Chemistry, Vol. 84, No. 10, 25.10.2012, p. 2027-2043.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform
AU - Guo, Shuai
AU - Nakano, Koji
AU - Nakajima, Hizuru
AU - Uchiyama, Katsumi
AU - Hemmi, Akihide
AU - Yamasaki, Yoshikazu
AU - Morooka, Shigeharu
AU - Ishimatsu, Ryoichi
AU - Imato, Toshihiko
PY - 2012/10/25
Y1 - 2012/10/25
N2 - A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.
AB - A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.
UR - http://www.scopus.com/inward/record.url?scp=84867669800&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867669800&partnerID=8YFLogxK
U2 - 10.1351/PAC-CON-11-10-10
DO - 10.1351/PAC-CON-11-10-10
M3 - Article
AN - SCOPUS:84867669800
VL - 84
SP - 2027
EP - 2043
JO - Pure and Applied Chemistry
JF - Pure and Applied Chemistry
SN - 0033-4545
IS - 10
ER -