Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo; Koji Nakano; Hizuru Nakajima; Katsumi Uchiyama; Akihide Hemmi; Yoshikazu Yamasaki; Shigeharu Morooka; Ryoichi Ishimatsu; Toshihiko Imato

doi:10.1351/PAC-CON-11-10-10

Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo, Koji Nakano, Hizuru Nakajima, Katsumi Uchiyama, Akihide Hemmi, Yoshikazu Yamasaki, Shigeharu Morooka, Ryoichi Ishimatsu, Toshihiko Imato

Molecular Information Chemistry

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H ₂O ₂ and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC ₈₀.

Original language	English
Pages (from-to)	2027-2043
Number of pages	17
Journal	Pure and Applied Chemistry
Volume	84
Issue number	10
DOIs	https://doi.org/10.1351/PAC-CON-11-10-10
Publication status	Published - Oct 25 2012

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

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Guo, S., Nakano, K., Nakajima, H., Uchiyama, K., Hemmi, A., Yamasaki, Y., Morooka, S., Ishimatsu, R., & Imato, T. (2012). Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform. Pure and Applied Chemistry, 84(10), 2027-2043. https://doi.org/10.1351/PAC-CON-11-10-10

Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform. / Guo, Shuai; Nakano, Koji; Nakajima, Hizuru; Uchiyama, Katsumi; Hemmi, Akihide; Yamasaki, Yoshikazu; Morooka, Shigeharu; Ishimatsu, Ryoichi; Imato, Toshihiko.

In: Pure and Applied Chemistry, Vol. 84, No. 10, 25.10.2012, p. 2027-2043.

Research output: Contribution to journal › Article

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AU - Nakano, Koji

AU - Nakajima, Hizuru

AU - Uchiyama, Katsumi

AU - Hemmi, Akihide

AU - Yamasaki, Yoshikazu

AU - Morooka, Shigeharu

AU - Ishimatsu, Ryoichi

AU - Imato, Toshihiko

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