Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo, Koji Nakano, Hizuru Nakajima, Katsumi Uchiyama, Akihide Hemmi, Yoshikazu Yamasaki, Shigeharu Morooka, Ryoichi Ishimatsu, Toshihiko Imato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.

Original languageEnglish
Pages (from-to)2027-2043
Number of pages17
JournalPure and Applied Chemistry
Volume84
Issue number10
DOIs
Publication statusPublished - Oct 25 2012

Fingerprint

Chemiluminescence
Nonionic surfactants
Microfluidics
Immobilized Antibodies
Luminol
Horseradish Peroxidase
Antibodies
Pumps

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform. / Guo, Shuai; Nakano, Koji; Nakajima, Hizuru; Uchiyama, Katsumi; Hemmi, Akihide; Yamasaki, Yoshikazu; Morooka, Shigeharu; Ishimatsu, Ryoichi; Imato, Toshihiko.

In: Pure and Applied Chemistry, Vol. 84, No. 10, 25.10.2012, p. 2027-2043.

Research output: Contribution to journalArticle

Guo, Shuai ; Nakano, Koji ; Nakajima, Hizuru ; Uchiyama, Katsumi ; Hemmi, Akihide ; Yamasaki, Yoshikazu ; Morooka, Shigeharu ; Ishimatsu, Ryoichi ; Imato, Toshihiko. / Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform. In: Pure and Applied Chemistry. 2012 ; Vol. 84, No. 10. pp. 2027-2043.
@article{22e4276574bc4e05904cc482b89c5eac,
title = "Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform",
abstract = "A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.",
author = "Shuai Guo and Koji Nakano and Hizuru Nakajima and Katsumi Uchiyama and Akihide Hemmi and Yoshikazu Yamasaki and Shigeharu Morooka and Ryoichi Ishimatsu and Toshihiko Imato",
year = "2012",
month = "10",
day = "25",
doi = "10.1351/PAC-CON-11-10-10",
language = "English",
volume = "84",
pages = "2027--2043",
journal = "Pure and Applied Chemistry",
issn = "0033-4545",
publisher = "IUPAC Secretariat",
number = "10",

}

TY - JOUR

T1 - Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

AU - Guo, Shuai

AU - Nakano, Koji

AU - Nakajima, Hizuru

AU - Uchiyama, Katsumi

AU - Hemmi, Akihide

AU - Yamasaki, Yoshikazu

AU - Morooka, Shigeharu

AU - Ishimatsu, Ryoichi

AU - Imato, Toshihiko

PY - 2012/10/25

Y1 - 2012/10/25

N2 - A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.

AB - A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.

UR - http://www.scopus.com/inward/record.url?scp=84867669800&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867669800&partnerID=8YFLogxK

U2 - 10.1351/PAC-CON-11-10-10

DO - 10.1351/PAC-CON-11-10-10

M3 - Article

AN - SCOPUS:84867669800

VL - 84

SP - 2027

EP - 2043

JO - Pure and Applied Chemistry

JF - Pure and Applied Chemistry

SN - 0033-4545

IS - 10

ER -