Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo, Koji Nakano, Hizuru Nakajima, Katsumi Uchiyama, Akihide Hemmi, Yoshikazu Yamasaki, Shigeharu Morooka, Ryoichi Ishimatsu, Toshihiko Imato

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.

    Original languageEnglish
    Pages (from-to)2027-2043
    Number of pages17
    JournalPure and Applied Chemistry
    Volume84
    Issue number10
    DOIs
    Publication statusPublished - 2012

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)
    • Chemical Engineering(all)

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