Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo, Koji Nakano, Hizuru Nakajima, Katsumi Uchiyama, Akihide Hemmi, Yoshikazu Yamasaki, Shigeharu Morooka, Ryoichi Ishimatsu, Toshihiko Imato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.

Original languageEnglish
Pages (from-to)2027-2043
Number of pages17
JournalPure and Applied Chemistry
Volume84
Issue number10
DOIs
Publication statusPublished - Oct 25 2012

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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