Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates

Manabu Nakazono, Hitoshi Nohta, Kazumi Sasamoto, Yosuke Ohkura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-f3-D-galactoside and o-aminophthalylhydrazide-0-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for 13-D-galactosidase and 100 for alkaline phosphatase.

Original languageEnglish
Pages (from-to)779-783
Number of pages5
Journalanalytical sciences
Volume8
Issue number6
DOIs
Publication statusPublished - Jan 1 1992

Fingerprint

Galactosidases
Luminol
Alkaline Phosphatase
Assays
Derivatives
Galactosides
Enzyme activity
Substrates
Phosphates
potassium ferricyanide

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates. / Nakazono, Manabu; Nohta, Hitoshi; Sasamoto, Kazumi; Ohkura, Yosuke.

In: analytical sciences, Vol. 8, No. 6, 01.01.1992, p. 779-783.

Research output: Contribution to journalArticle

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