Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates

Manabu Nakazono, Hitoshi Nohta, Kazumi Sasamoto, Yosuke Ohkura

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Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-f3-D-galactoside and o-aminophthalylhydrazide-0-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for 13-D-galactosidase and 100 for alkaline phosphatase.

Original languageEnglish
Pages (from-to)779-783
Number of pages5
Journalanalytical sciences
Issue number6
Publication statusPublished - Jan 1 1992


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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