Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates

Manabu Nakazono; Hitoshi Nohta; Kazumi Sasamoto; Yosuke Ohkura

doi:10.2116/analsci.8.779

Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates

Manabu Nakazono, Hitoshi Nohta, Kazumi Sasamoto, Yosuke Ohkura

Molecular Bioinformatics

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-f3-D-galactoside and o-aminophthalylhydrazide-0-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for 13-D-galactosidase and 100 for alkaline phosphatase.

Original language	English
Pages (from-to)	779-783
Number of pages	5
Journal	analytical sciences
Volume	8
Issue number	6
DOIs	https://doi.org/10.2116/analsci.8.779
Publication status	Published - Jan 1 1992

All Science Journal Classification (ASJC) codes

Analytical Chemistry

Access to Document

10.2116/analsci.8.779

Cite this

@article{c0bb3e6a56a34d86bc3e77d5657522a5,

title = "Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates",

abstract = "Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-f3-D-galactoside and o-aminophthalylhydrazide-0-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for 13-D-galactosidase and 100 for alkaline phosphatase.",

author = "Manabu Nakazono and Hitoshi Nohta and Kazumi Sasamoto and Yosuke Ohkura",

year = "1992",

month = jan,

day = "1",

doi = "10.2116/analsci.8.779",

language = "English",

volume = "8",

pages = "779--783",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "The Japan Society for Analytical Chemistry",

number = "6",

}

TY - JOUR

T1 - Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates

AU - Nakazono, Manabu

AU - Nohta, Hitoshi

AU - Sasamoto, Kazumi

AU - Ohkura, Yosuke

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-f3-D-galactoside and o-aminophthalylhydrazide-0-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for 13-D-galactosidase and 100 for alkaline phosphatase.

AB - Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-f3-D-galactoside and o-aminophthalylhydrazide-0-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for 13-D-galactosidase and 100 for alkaline phosphatase.

UR - http://www.scopus.com/inward/record.url?scp=85007664408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007664408&partnerID=8YFLogxK

U2 - 10.2116/analsci.8.779

DO - 10.2116/analsci.8.779

M3 - Article

AN - SCOPUS:85007664408

SN - 0910-6340

VL - 8

SP - 779

EP - 783

JO - Analytical Sciences

JF - Analytical Sciences

IS - 6

ER -

Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase using Novel Luminol Derivatives as Substrates

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this