Chemosensitivity testing of human mouth carcinoma cell line

Takuya Tachikawa, Hirobumi Kumazawa, Yoshio Hori, Shinichi Sai, Jun Kita, Toshio Yamashita, Tadami Kumazawa, Yoshihiko Maehara, Shunji Kohnoe

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Abstract

The chemosensitivity of KB cells derived from oral epidermal carcinoma to various antitumor agents was analyzed using the MTT 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-2H tetrazolium bromide) assay. Optical density (OD) for MTT assay was measured with dual wavelengths. The chemosensitivity of the drugs was evaluated by the 50% OD (OD50) of each drug concentration in the control group. Five platinum (Pt) drugs and 3 anthracycline (AC) drugs were used in this study. The chemosensitivity differed among the 5 Pt drugs. No significant difference was observed among the 3 AC drugs. A linear increase in OD corresponding to an increase in number of cells was observed. When 0.1 M sodium succinate (S.S.) was added to 0.4% MTT, the sensitivity increased five-fold compared to the control group without S.S. The MTT assay is a precise, rapid, easy and inexpensive experimental system useful for evaluation of antitumor drug sensitivity on tumor cell lines.

Original languageEnglish
Pages (from-to)154-157
Number of pages4
JournalActa Oto-Laryngologica
Volume113
Issue numberS500
DOIs
Publication statusPublished - 1993

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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    Tachikawa, T., Kumazawa, H., Hori, Y., Sai, S., Kita, J., Yamashita, T., Kumazawa, T., Maehara, Y., & Kohnoe, S. (1993). Chemosensitivity testing of human mouth carcinoma cell line. Acta Oto-Laryngologica, 113(S500), 154-157. https://doi.org/10.3109/00016489309126201