Abstract
The chemosensitivity of KB cells derived from oral epidermal carcinoma to various antitumor agents was analyzed using the MTT 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-2H tetrazolium bromide) assay. Optical density (OD) for MTT assay was measured with dual wavelengths. The chemosensitivity of the drugs was evaluated by the 50% OD (OD50) of each drug concentration in the control group. Five platinum (Pt) drugs and 3 anthracycline (AC) drugs were used in this study. The chemosensitivity differed among the 5 Pt drugs. No significant difference was observed among the 3 AC drugs. A linear increase in OD corresponding to an increase in number of cells was observed. When 0.1 M sodium succinate (S.S.) was added to 0.4% MTT, the sensitivity increased five-fold compared to the control group without S.S. The MTT assay is a precise, rapid, easy and inexpensive experimental system useful for evaluation of antitumor drug sensitivity on tumor cell lines.
| Original language | English |
|---|---|
| Pages (from-to) | 154-157 |
| Number of pages | 4 |
| Journal | Acta Oto-Laryngologica |
| Volume | 113 |
| Issue number | S500 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
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All Science Journal Classification (ASJC) codes
- Otorhinolaryngology
Cite this
Chemosensitivity testing of human mouth carcinoma cell line. / Tachikawa, Takuya; Kumazawa, Hirobumi; Hori, Yoshio; Sai, Shinichi; Kita, Jun; Yamashita, Toshio; Kumazawa, Tadami; Maehara, Yoshihiko; Kohnoe, Shunji.
In: Acta Oto-Laryngologica, Vol. 113, No. S500, 01.01.1993, p. 154-157.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemosensitivity testing of human mouth carcinoma cell line
AU - Tachikawa, Takuya
AU - Kumazawa, Hirobumi
AU - Hori, Yoshio
AU - Sai, Shinichi
AU - Kita, Jun
AU - Yamashita, Toshio
AU - Kumazawa, Tadami
AU - Maehara, Yoshihiko
AU - Kohnoe, Shunji
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The chemosensitivity of KB cells derived from oral epidermal carcinoma to various antitumor agents was analyzed using the MTT 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-2H tetrazolium bromide) assay. Optical density (OD) for MTT assay was measured with dual wavelengths. The chemosensitivity of the drugs was evaluated by the 50% OD (OD50) of each drug concentration in the control group. Five platinum (Pt) drugs and 3 anthracycline (AC) drugs were used in this study. The chemosensitivity differed among the 5 Pt drugs. No significant difference was observed among the 3 AC drugs. A linear increase in OD corresponding to an increase in number of cells was observed. When 0.1 M sodium succinate (S.S.) was added to 0.4% MTT, the sensitivity increased five-fold compared to the control group without S.S. The MTT assay is a precise, rapid, easy and inexpensive experimental system useful for evaluation of antitumor drug sensitivity on tumor cell lines.
AB - The chemosensitivity of KB cells derived from oral epidermal carcinoma to various antitumor agents was analyzed using the MTT 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-2H tetrazolium bromide) assay. Optical density (OD) for MTT assay was measured with dual wavelengths. The chemosensitivity of the drugs was evaluated by the 50% OD (OD50) of each drug concentration in the control group. Five platinum (Pt) drugs and 3 anthracycline (AC) drugs were used in this study. The chemosensitivity differed among the 5 Pt drugs. No significant difference was observed among the 3 AC drugs. A linear increase in OD corresponding to an increase in number of cells was observed. When 0.1 M sodium succinate (S.S.) was added to 0.4% MTT, the sensitivity increased five-fold compared to the control group without S.S. The MTT assay is a precise, rapid, easy and inexpensive experimental system useful for evaluation of antitumor drug sensitivity on tumor cell lines.
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U2 - 10.3109/00016489309126201
DO - 10.3109/00016489309126201
M3 - Article
C2 - 8452015
AN - SCOPUS:0027345362
VL - 113
SP - 154
EP - 157
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
SN - 0001-6489
IS - S500
ER -