TY - JOUR
T1 - Chemosensitivity Testing with MTT Assay Using a Human Mouth Carcinoma Cell Line
AU - Tachikawa, Takuya
AU - Kumazawa, Hirobumi
AU - Hori, Yoshio
AU - Sai, Shinichi
AU - Kita, Jun
AU - Wada, Yasuhiro
AU - Yamashita, Toshio
AU - Kumazawa, Tadami
AU - Maehara, Yoshihiko
AU - Kohnoe, Shunji
PY - 1993
Y1 - 1993
N2 - We performed chemosensitivity tests with MTT assay using KB cells derived from epidermal carcinoma of the mouth. Optical density (OD) for the MTT assay was measured with dual wave lengths: measurement wave (540 nm) and reference wave (620 nm). Five platinum (Pt) drugs and three anthracycline drugs were tested. The chemosen sitivity to drugs was evaluated by the 50% OD of each drug concentration in the control group. CDDP was the most effective Pt drug, followed by 254S, NK121, CBDCA and DWA2114R. No significant difference among the three anthracycline drugs. We observ ed a linear increase of OD, corresponding to the increase in the number of cells. When we added 0.1 M sodium succinate (SS) to 0.4% MTT {3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide}, the sensitivity of this assay was increased five fold. MTT assay is a precise, rapid, easy and inexpensive experimental system, which should be an useful method of evaluating the effect of antitumor drugs on tumor cell lines.
AB - We performed chemosensitivity tests with MTT assay using KB cells derived from epidermal carcinoma of the mouth. Optical density (OD) for the MTT assay was measured with dual wave lengths: measurement wave (540 nm) and reference wave (620 nm). Five platinum (Pt) drugs and three anthracycline drugs were tested. The chemosen sitivity to drugs was evaluated by the 50% OD of each drug concentration in the control group. CDDP was the most effective Pt drug, followed by 254S, NK121, CBDCA and DWA2114R. No significant difference among the three anthracycline drugs. We observ ed a linear increase of OD, corresponding to the increase in the number of cells. When we added 0.1 M sodium succinate (SS) to 0.4% MTT {3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide}, the sensitivity of this assay was increased five fold. MTT assay is a precise, rapid, easy and inexpensive experimental system, which should be an useful method of evaluating the effect of antitumor drugs on tumor cell lines.
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U2 - 10.5631/jibirin.86.129
DO - 10.5631/jibirin.86.129
M3 - Article
AN - SCOPUS:0027514589
SN - 0032-6313
VL - 86
SP - 129
EP - 134
JO - Practica Otologica
JF - Practica Otologica
IS - 1
ER -