Chemosensitivity Testing with MTT Assay Using a Human Mouth Carcinoma Cell Line

Takuya Tachikawa, Hirobumi Kumazawa, Yoshio Hori, Shinichi Sai, Jun Kita, Yasuhiro Wada, Toshio Yamashita, Tadami Kumazawa, Yoshihiko Maehara, Shunji Kohnoe

Research output: Contribution to journalArticlepeer-review

Abstract

We performed chemosensitivity tests with MTT assay using KB cells derived from epidermal carcinoma of the mouth. Optical density (OD) for the MTT assay was measured with dual wave lengths: measurement wave (540 nm) and reference wave (620 nm). Five platinum (Pt) drugs and three anthracycline drugs were tested. The chemosen sitivity to drugs was evaluated by the 50% OD of each drug concentration in the control group. CDDP was the most effective Pt drug, followed by 254S, NK121, CBDCA and DWA2114R. No significant difference among the three anthracycline drugs. We observ ed a linear increase of OD, corresponding to the increase in the number of cells. When we added 0.1 M sodium succinate (SS) to 0.4% MTT {3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide}, the sensitivity of this assay was increased five fold. MTT assay is a precise, rapid, easy and inexpensive experimental system, which should be an useful method of evaluating the effect of antitumor drugs on tumor cell lines.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalPractica Otologica
Volume86
Issue number1
DOIs
Publication statusPublished - 1993

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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