Chimeric fluorescent fusion proteins to monitor autophagy in plants.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Autophagy is induced under nutrient-deficient conditions in both growing tobacco BY-2 cultured cells as well as Arabidopsis and others intact plants. The fluorescent protein-tagged structural protein for autophagosomes, the Atg8 protein, allows nondestructive detection of autophagy induction in plant cells and tissues by fluorescence microscopy. Using this technique, the general operation of autophagy in growing root cells has been observed. A synthetic cargo protein for autophagy consisting of cytochrome b5 and the red fluorescence protein, DsRed, allows for the quantitative assay of autophagy in tobacco cells. This chapter describes methods for detecting autophagy in these plant cells using fluorescent protein fusions in situ with light microscopy, as well as quantification of autophagy.

Original languageEnglish
Pages (from-to)541-555
Number of pages15
JournalMethods in Enzymology
Volume451
Publication statusPublished - Dec 1 2008

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Autophagy
Fusion reactions
Proteins
Tobacco
Plant Cells
Cytochromes b5
Fluorescence microscopy
Nutrients
Optical microscopy
Assays
Fluorescence Microscopy
Arabidopsis
Fluorescence
Cells
Microscopy
Cultured Cells
Tissue
Light
Food

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Chimeric fluorescent fusion proteins to monitor autophagy in plants. / Matsuoka, Ken.

In: Methods in Enzymology, Vol. 451, 01.12.2008, p. 541-555.

Research output: Contribution to journalArticle

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