Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice

Shiro Kitamoto, Kensuke Egashira, Toshihiro Ichiki, Xinbing Han, Sara McCurdy, Shohei Sakuda, Kenji Sunagawa, William A. Boisvert

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Chitinase 1 (CHIT1) is secreted by activated macrophages. Chitinase activity is raised in atherosclerotic patient sera and is present in atherosclerotic plaque. However, the role of CHIT1 in atherosclerosis is unknown. Preliminary studies of atherosclerosis in cynomolgous monkeys revealed CHIT1 to be closely correlated with areas of macrophage infiltration. Thus, we investigated the effects of a chitinase inhibitor, allosamidin, on macrophage function in vitro and on atherosclerotic development in vivo. In RAW264.7 cells, allosamidin elevated monocyte chemoattractant protein 1 and tumor necrosis factor alpha expression, and increased activator protein 1 and nuclear factor-κB transcriptional activity. Although inducible nitric oxide synthase, IL-6, and IL-1β expression were increased, Arg1 expression was decreased by chitinase inhibition, suggesting that suppression of CHIT1 activity polarizes macrophages into a M1 phenotype. Allosamidin decreased scavenger receptor AI, CD36, ABCA1, and ABCG1 expression which led to suppression of cholesterol uptake and apolipoprotein AI-mediated cholesterol efflux in macrophages. These effects were confirmed with CHIT1 siRNA transfection and CHIT1 plasmid transfection experiments in primary macrophages. Apolipoprotein E-deficient hyperlipidemic mice treated for 6 weeks with constant administration of allosamidin and fed an atherogenic diet showed aggravated atherosclerotic lesion formation. These data suggest that CHIT1 exerts protective effects against atherosclerosis by suppressing inflammatory responses and polarizing macrophages toward an M2 phenotype, and promoting lipid uptake and cholesterol efflux in macrophages.

Original languageEnglish
Pages (from-to)313-325
Number of pages13
JournalAmerican Journal of Pathology
Volume183
Issue number1
DOIs
Publication statusPublished - Jul 1 2013

Fingerprint

Chitinases
allosamidin
Atherosclerosis
Macrophages
Cholesterol
Transfection
Atherogenic Diet
Phenotype
Scavenger Receptors
Chemokine CCL2
Apolipoprotein A-I
Transcription Factor AP-1
Apolipoproteins E
Nitric Oxide Synthase Type II
Atherosclerotic Plaques
Interleukin-1
Small Interfering RNA
Haplorhini
Interleukin-6
Plasmids

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Kitamoto, S., Egashira, K., Ichiki, T., Han, X., McCurdy, S., Sakuda, S., ... Boisvert, W. A. (2013). Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice. American Journal of Pathology, 183(1), 313-325. https://doi.org/10.1016/j.ajpath.2013.04.003

Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice. / Kitamoto, Shiro; Egashira, Kensuke; Ichiki, Toshihiro; Han, Xinbing; McCurdy, Sara; Sakuda, Shohei; Sunagawa, Kenji; Boisvert, William A.

In: American Journal of Pathology, Vol. 183, No. 1, 01.07.2013, p. 313-325.

Research output: Contribution to journalArticle

Kitamoto, S, Egashira, K, Ichiki, T, Han, X, McCurdy, S, Sakuda, S, Sunagawa, K & Boisvert, WA 2013, 'Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice', American Journal of Pathology, vol. 183, no. 1, pp. 313-325. https://doi.org/10.1016/j.ajpath.2013.04.003
Kitamoto, Shiro ; Egashira, Kensuke ; Ichiki, Toshihiro ; Han, Xinbing ; McCurdy, Sara ; Sakuda, Shohei ; Sunagawa, Kenji ; Boisvert, William A. / Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice. In: American Journal of Pathology. 2013 ; Vol. 183, No. 1. pp. 313-325.
@article{ac8a9a2f7fd74b458293566365943cc0,
title = "Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice",
abstract = "Chitinase 1 (CHIT1) is secreted by activated macrophages. Chitinase activity is raised in atherosclerotic patient sera and is present in atherosclerotic plaque. However, the role of CHIT1 in atherosclerosis is unknown. Preliminary studies of atherosclerosis in cynomolgous monkeys revealed CHIT1 to be closely correlated with areas of macrophage infiltration. Thus, we investigated the effects of a chitinase inhibitor, allosamidin, on macrophage function in vitro and on atherosclerotic development in vivo. In RAW264.7 cells, allosamidin elevated monocyte chemoattractant protein 1 and tumor necrosis factor alpha expression, and increased activator protein 1 and nuclear factor-κB transcriptional activity. Although inducible nitric oxide synthase, IL-6, and IL-1β expression were increased, Arg1 expression was decreased by chitinase inhibition, suggesting that suppression of CHIT1 activity polarizes macrophages into a M1 phenotype. Allosamidin decreased scavenger receptor AI, CD36, ABCA1, and ABCG1 expression which led to suppression of cholesterol uptake and apolipoprotein AI-mediated cholesterol efflux in macrophages. These effects were confirmed with CHIT1 siRNA transfection and CHIT1 plasmid transfection experiments in primary macrophages. Apolipoprotein E-deficient hyperlipidemic mice treated for 6 weeks with constant administration of allosamidin and fed an atherogenic diet showed aggravated atherosclerotic lesion formation. These data suggest that CHIT1 exerts protective effects against atherosclerosis by suppressing inflammatory responses and polarizing macrophages toward an M2 phenotype, and promoting lipid uptake and cholesterol efflux in macrophages.",
author = "Shiro Kitamoto and Kensuke Egashira and Toshihiro Ichiki and Xinbing Han and Sara McCurdy and Shohei Sakuda and Kenji Sunagawa and Boisvert, {William A.}",
year = "2013",
month = "7",
day = "1",
doi = "10.1016/j.ajpath.2013.04.003",
language = "English",
volume = "183",
pages = "313--325",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Chitinase inhibition promotes atherosclerosis in hyperlipidemic mice

AU - Kitamoto, Shiro

AU - Egashira, Kensuke

AU - Ichiki, Toshihiro

AU - Han, Xinbing

AU - McCurdy, Sara

AU - Sakuda, Shohei

AU - Sunagawa, Kenji

AU - Boisvert, William A.

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Chitinase 1 (CHIT1) is secreted by activated macrophages. Chitinase activity is raised in atherosclerotic patient sera and is present in atherosclerotic plaque. However, the role of CHIT1 in atherosclerosis is unknown. Preliminary studies of atherosclerosis in cynomolgous monkeys revealed CHIT1 to be closely correlated with areas of macrophage infiltration. Thus, we investigated the effects of a chitinase inhibitor, allosamidin, on macrophage function in vitro and on atherosclerotic development in vivo. In RAW264.7 cells, allosamidin elevated monocyte chemoattractant protein 1 and tumor necrosis factor alpha expression, and increased activator protein 1 and nuclear factor-κB transcriptional activity. Although inducible nitric oxide synthase, IL-6, and IL-1β expression were increased, Arg1 expression was decreased by chitinase inhibition, suggesting that suppression of CHIT1 activity polarizes macrophages into a M1 phenotype. Allosamidin decreased scavenger receptor AI, CD36, ABCA1, and ABCG1 expression which led to suppression of cholesterol uptake and apolipoprotein AI-mediated cholesterol efflux in macrophages. These effects were confirmed with CHIT1 siRNA transfection and CHIT1 plasmid transfection experiments in primary macrophages. Apolipoprotein E-deficient hyperlipidemic mice treated for 6 weeks with constant administration of allosamidin and fed an atherogenic diet showed aggravated atherosclerotic lesion formation. These data suggest that CHIT1 exerts protective effects against atherosclerosis by suppressing inflammatory responses and polarizing macrophages toward an M2 phenotype, and promoting lipid uptake and cholesterol efflux in macrophages.

AB - Chitinase 1 (CHIT1) is secreted by activated macrophages. Chitinase activity is raised in atherosclerotic patient sera and is present in atherosclerotic plaque. However, the role of CHIT1 in atherosclerosis is unknown. Preliminary studies of atherosclerosis in cynomolgous monkeys revealed CHIT1 to be closely correlated with areas of macrophage infiltration. Thus, we investigated the effects of a chitinase inhibitor, allosamidin, on macrophage function in vitro and on atherosclerotic development in vivo. In RAW264.7 cells, allosamidin elevated monocyte chemoattractant protein 1 and tumor necrosis factor alpha expression, and increased activator protein 1 and nuclear factor-κB transcriptional activity. Although inducible nitric oxide synthase, IL-6, and IL-1β expression were increased, Arg1 expression was decreased by chitinase inhibition, suggesting that suppression of CHIT1 activity polarizes macrophages into a M1 phenotype. Allosamidin decreased scavenger receptor AI, CD36, ABCA1, and ABCG1 expression which led to suppression of cholesterol uptake and apolipoprotein AI-mediated cholesterol efflux in macrophages. These effects were confirmed with CHIT1 siRNA transfection and CHIT1 plasmid transfection experiments in primary macrophages. Apolipoprotein E-deficient hyperlipidemic mice treated for 6 weeks with constant administration of allosamidin and fed an atherogenic diet showed aggravated atherosclerotic lesion formation. These data suggest that CHIT1 exerts protective effects against atherosclerosis by suppressing inflammatory responses and polarizing macrophages toward an M2 phenotype, and promoting lipid uptake and cholesterol efflux in macrophages.

UR - http://www.scopus.com/inward/record.url?scp=84879397603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879397603&partnerID=8YFLogxK

U2 - 10.1016/j.ajpath.2013.04.003

DO - 10.1016/j.ajpath.2013.04.003

M3 - Article

VL - 183

SP - 313

EP - 325

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 1

ER -