Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and methane-grown Methylosinus trichosporium OB3b

Natsuko Hamamura, Cynthia Page, Tulley Long, Lewis Semprini, Daniel J. Arp

Research output: Contribution to journalArticle

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Abstract

Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader Methylosinus trichosporium OB3b. All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. CF degradation by all four bacteria required O2. Butane inhibited CF degradation by the butane-grown bacteria, suggesting that butane monooxygenase is responsible for CF degradation. P. butanovora required exogenous reductant to degrade CF, while CF8 and M. vaccae utilized endogenous reductants. Prolonged incubation with CF resulted in decreased CF degradation. CF8 and P. butanovora were more sensitive to CF than either M. trichosporium or M. vaccae. CF degradation by all three butane-grown bacteria was in-activated by acetylene, which is a mechanism- based inhibitor for several monooxygenases. Butane protected all three butane-grown bacteria from inactivation by acetylene, which indicates that the same monooxygenase is responsible for both CF and butane oxidation. CF8 and P. butanovora were able to degrade other chlorinated hydrocarbons, including trichloroethylene, 1,2-cis-dichloroethylene, and vinyl chloride. In addition, CF8 degraded 1,1,2-trichloroethane. The results indicate the potential of butane-grown bacteria for chlorinated hydro-carbon transformation.

Original languageEnglish
Pages (from-to)3607-3613
Number of pages7
JournalApplied and environmental microbiology
Volume63
Issue number9
Publication statusPublished - Sep 1 1997

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Pseudomonas butanovora
Methylosinus trichosporium
Mycobacterium vaccae
Methane
Mycobacterium
Chloroform
chloroform
Pseudomonas
methane
Bacteria
degradation
bacterium
bacteria
Mixed Function Oxygenases
Acetylene
acetylene
reducing agents
Reducing Agents
butanes
butane

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and methane-grown Methylosinus trichosporium OB3b. / Hamamura, Natsuko; Page, Cynthia; Long, Tulley; Semprini, Lewis; Arp, Daniel J.

In: Applied and environmental microbiology, Vol. 63, No. 9, 01.09.1997, p. 3607-3613.

Research output: Contribution to journalArticle

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