Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs

Takahiro Nakamura, Masaru Ohta, Masahiro Sugiura, Mamoru Sugita

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts. Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron-containing precursor (pre-) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre-tRNA splicing.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalFEBS Letters
Volume460
Issue number3
DOIs
Publication statusPublished - Nov 5 1999
Externally publishedYes

Fingerprint

Ribonucleoproteins
RNA Precursors
Chloroplasts
Introns
Messenger RNA
Tobacco
Nucleic Acids
RNA Stability
RNA
Immunoprecipitation
Molecules
Processing

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs. / Nakamura, Takahiro; Ohta, Masaru; Sugiura, Masahiro; Sugita, Mamoru.

In: FEBS Letters, Vol. 460, No. 3, 05.11.1999, p. 437-441.

Research output: Contribution to journalArticle

Nakamura, Takahiro ; Ohta, Masaru ; Sugiura, Masahiro ; Sugita, Mamoru. / Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs. In: FEBS Letters. 1999 ; Vol. 460, No. 3. pp. 437-441.
@article{7fb27ab99d0f44ecad7f80c96546dd2f,
title = "Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs",
abstract = "Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts. Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron-containing precursor (pre-) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre-tRNA splicing.",
author = "Takahiro Nakamura and Masaru Ohta and Masahiro Sugiura and Mamoru Sugita",
year = "1999",
month = "11",
day = "5",
doi = "10.1016/S0014-5793(99)01390-3",
language = "English",
volume = "460",
pages = "437--441",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs

AU - Nakamura, Takahiro

AU - Ohta, Masaru

AU - Sugiura, Masahiro

AU - Sugita, Mamoru

PY - 1999/11/5

Y1 - 1999/11/5

N2 - Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts. Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron-containing precursor (pre-) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre-tRNA splicing.

AB - Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts. Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron-containing precursor (pre-) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre-tRNA splicing.

UR - http://www.scopus.com/inward/record.url?scp=0032755049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032755049&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)01390-3

DO - 10.1016/S0014-5793(99)01390-3

M3 - Article

C2 - 10556512

AN - SCOPUS:0032755049

VL - 460

SP - 437

EP - 441

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -