Chloroplast ribonucleoproteins (RNPs) as phosphate acceptors for casein kinase II: Purification by ssDNA-cellulose column chromatography

Motoki Kanekatsu, Akiyoshi Ezumi, Takahiro Nakamura, Kenzo Ohtsuki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Using ssDNA-cellulose column chromatography, a 34 kDa ribonucleoprotein (p34) has been purified from a 0.4 M KCl crude extract of spinach chloroplasts as an effective phosphate acceptor for casein kinase II (CK-II) in vitro. Monomeric and oligomeric CK-IIs were copurified with p34 by the column chromatography and the kinases were separated from p34 by means of Mono Q column chromatography. It was found that (i) the purified p34 (pi 4.9) was phosphorylated specifically by CK-II in vitro; and (ii) similar polypeptides, such as p35 (pI 4.7) and p39 (pI 4.9) in maize and p33 (pI 4.7) in liverwort, were detected as ssDNA-binding chloroplast proteins phosphorylated by CK-II in vitro. The findings suggest that (i) RNPs that function as phosphate acceptors for CK-II exist commonly in chloroplasts among plant cells; and (ii) the physiological activity of RNPs is regulated by their specific phosphorylation by CK-II in chloroplasts.

Original languageEnglish
Pages (from-to)1649-1656
Number of pages8
JournalPlant and Cell Physiology
Volume36
Issue number8
Publication statusPublished - Dec 1 1995
Externally publishedYes

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Casein Kinase II
ribonucleoproteins
single-stranded DNA
Ribonucleoproteins
Chloroplasts
Cellulose
Chromatography
chromatography
cellulose
chloroplasts
Phosphates
phosphates
Hepatophyta
Chloroplast Proteins
Spinacia oleracea
Plant Cells
Complex Mixtures
spinach
Zea mays
mosses and liverworts

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Chloroplast ribonucleoproteins (RNPs) as phosphate acceptors for casein kinase II : Purification by ssDNA-cellulose column chromatography. / Kanekatsu, Motoki; Ezumi, Akiyoshi; Nakamura, Takahiro; Ohtsuki, Kenzo.

In: Plant and Cell Physiology, Vol. 36, No. 8, 01.12.1995, p. 1649-1656.

Research output: Contribution to journalArticle

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