Chloroplast RNA-binding and pentatricopeptide repeat proteins

Takahiro Nakamura, G. Schuster, M. Sugiura, M. Sugita

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Chloroplast gene expression is mainly regulated at the post-transcriptional level by numerous nuclear-encoded RNA-binding protein factors. In the present study, we focus on two RNA-binding proteins: cpRNP (chloroplast ribonucleoprotein) and PPR (pentatricopeptide repeat) protein. These are suggested to be major contributors to chloroplast RNA metabolism. Tobacco cpRNPs are composed of five different proteins containing two RNA-recognition motifs and an acidic N-terminal domain. The cpRNPs are abundant proteins and form heterogeneous complexes with most ribosome-free mRNAs and the precursors of tRNAs in the stroma. The complexes could function as platforms for various RNA-processing events in chloroplasts. It has been demonstrated that cpRNPs contribute to RNA stabilization, 3′-end formation and editing. The PPR proteins occur as a superfamily only in the higher plant species. They are predicted to be involved in RNA/DNA metabolism in chloroplasts or mitochondria. Nuclear-encoded HCF152 is a chloroplast-localized protein that usually has 12 PPR motifs. The null mutant of Arabidopsis, hcf152, is impaired in the 5′-end processing and splicing of petB transcripts. HCF152 binds the petB exon-intron junctions with high affinity. The number of PPR motifs controls its affinity and specificity for RNA. It has been suggested that each of the highly variable PPR proteins is a gene-specific regulator of plant organellar RNA metabolism.

Original languageEnglish
Pages (from-to)571-574
Number of pages4
JournalBiochemical Society Transactions
Volume32
Issue number4
DOIs
Publication statusPublished - Aug 1 2004

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Chloroplast RNA
RNA
Chloroplasts
Metabolism
RNA-Binding Proteins
Proteins
Plant RNA
Chloroplast Genes
Chloroplast Proteins
Nuclear RNA
Ribonucleoproteins
RNA Precursors
Regulator Genes
Transfer RNA
Mitochondria
Ribosomes
Arabidopsis
Tobacco
Introns
Processing

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Chloroplast RNA-binding and pentatricopeptide repeat proteins. / Nakamura, Takahiro; Schuster, G.; Sugiura, M.; Sugita, M.

In: Biochemical Society Transactions, Vol. 32, No. 4, 01.08.2004, p. 571-574.

Research output: Contribution to journalArticle

Nakamura, Takahiro ; Schuster, G. ; Sugiura, M. ; Sugita, M. / Chloroplast RNA-binding and pentatricopeptide repeat proteins. In: Biochemical Society Transactions. 2004 ; Vol. 32, No. 4. pp. 571-574.
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