Chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of rat tibial articular cartilage

Yasuyuki Sasano, Mitsuru Furusawa, Haruo Ohtani, Itaru Mizoguchi, Ichiro Takahashi, Manabu Kagayama

Research output: Contribution to journalArticle

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Abstract

The present study was designed to investigate whether or not chondrocytes in articular cartilage express type I collagen in vivo under physiological conditions. Expressions of the gene and the phenotype of type I collagen were examined in rat tibial articular cartilage in the knee joint during development. Knee joints of Wistar rats at 1, 5, and 11 weeks postnatal were fixed in 4% paraformaldehyde with or without 0.5% glutaraldehyde and decalcified in 10% EDTA. After the specimens were embedded in paraffin and serial sections made, adjacent sections were processed for immunohistochemistry and in situ hybridization for type I collagen. The epiphysis of the tibia was composed of cartilage in week 1 rats. Formation of articular cartilage was in progress in week 5 as endochondral ossification proceeded and was completed in week 11. Anti-type I collagen antibody stained only the superficial area of the epiphysis in week 1, but the immunoreactivity was expanded into the deeper region of the articular cartilage with development in weeks 5 and 11. Hybridization signals for pro-alpha 1 (I) collagen were seen in some of chondrocytes in the epiphysis of the week 1 tibia. The most intense signals were identified in chondrocytes in week 5 and the signals appeared weaker in week 11. The present study demonstrated that chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of the articular cartilage.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalAnatomy and Embryology
Volume194
Issue number3
Publication statusPublished - Sep 5 1996
Externally publishedYes

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Articular Cartilage
Chondrocytes
Collagen Type I
Epiphyses
Proteins
Knee Joint
Tibia
Glutaral
Osteogenesis
Edetic Acid
Paraffin
Cartilage
In Situ Hybridization
Wistar Rats
Immunohistochemistry
Phenotype
Gene Expression
Antibodies

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

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Chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of rat tibial articular cartilage. / Sasano, Yasuyuki; Furusawa, Mitsuru; Ohtani, Haruo; Mizoguchi, Itaru; Takahashi, Ichiro; Kagayama, Manabu.

In: Anatomy and Embryology, Vol. 194, No. 3, 05.09.1996, p. 247-252.

Research output: Contribution to journalArticle

Sasano, Yasuyuki ; Furusawa, Mitsuru ; Ohtani, Haruo ; Mizoguchi, Itaru ; Takahashi, Ichiro ; Kagayama, Manabu. / Chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of rat tibial articular cartilage. In: Anatomy and Embryology. 1996 ; Vol. 194, No. 3. pp. 247-252.
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