Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans

Souhei Mizuguchi, Toru Uyama, Hiroshi Kitagawa, Kazuko H. Nomura, Katsufumi Dejima, Keiko Gengyo-Ando, Shohei Mitani, Kazuyuki Sugahara, Kazuya Nomura

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Glycosaminoglycans such as heparan sulphate and chondroitin sulphate are extracellular sugar chains involved in intercellular signalling. Disruptions of genes encoding enzymes that mediate glycosaminoglycan biosynthesis have severe consequences in Drosophila and mice. Mutations in the Drosophila gene sugarless, which encodes a UDP-glucose dehydrogenase, impair developmental signalling through the Wnt family member Wingless, and signalling by the fibroblast growth factor and Hedgehog pathways. Heparan sulphate is involved in these pathways, but little is known about the involvement of chondroitin. Undersulphated and oversulphated chondroitin sulphate chains have been implicated in other biological processes, however, including adhesion of erythrocytes infected with malaria parasite to human placenta and regulation of neural development. To investigate chondroitin functions, we cloned a chondroitin synthase homologue of Caenorhabditis elegans and depleted expression of its product by RNA-mediated interference and deletion mutagenesis. Here we report that blocking chondroitin synthesis results in cytokinesis defects in early embryogenesis. Reversion of cytokinesis is often observed in chondroitin-depleted embryos, and cell division eventually stops, resulting in early embryonic death. Our findings show that chondroitin is required for embryonic cytokinesis and cell division.

Original languageEnglish
Pages (from-to)443-448
Number of pages6
JournalNature
Volume423
Issue number6938
DOIs
Publication statusPublished - May 22 2003

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Chondroitin
Caenorhabditis elegans
Proteoglycans
Cell Division
Cytokinesis
Heparitin Sulfate
Chondroitin Sulfates
Glycosaminoglycans
Drosophila
Uridine Diphosphate Glucose Dehydrogenase
Biological Phenomena
Hedgehogs
Fibroblast Growth Factors
RNA Interference
Mutagenesis
Placenta
Genes
Malaria
Embryonic Development
Parasites

All Science Journal Classification (ASJC) codes

  • General

Cite this

Mizuguchi, S., Uyama, T., Kitagawa, H., Nomura, K. H., Dejima, K., Gengyo-Ando, K., ... Nomura, K. (2003). Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans. Nature, 423(6938), 443-448. https://doi.org/10.1038/nature01635

Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans. / Mizuguchi, Souhei; Uyama, Toru; Kitagawa, Hiroshi; Nomura, Kazuko H.; Dejima, Katsufumi; Gengyo-Ando, Keiko; Mitani, Shohei; Sugahara, Kazuyuki; Nomura, Kazuya.

In: Nature, Vol. 423, No. 6938, 22.05.2003, p. 443-448.

Research output: Contribution to journalArticle

Mizuguchi, S, Uyama, T, Kitagawa, H, Nomura, KH, Dejima, K, Gengyo-Ando, K, Mitani, S, Sugahara, K & Nomura, K 2003, 'Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans', Nature, vol. 423, no. 6938, pp. 443-448. https://doi.org/10.1038/nature01635
Mizuguchi S, Uyama T, Kitagawa H, Nomura KH, Dejima K, Gengyo-Ando K et al. Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans. Nature. 2003 May 22;423(6938):443-448. https://doi.org/10.1038/nature01635
Mizuguchi, Souhei ; Uyama, Toru ; Kitagawa, Hiroshi ; Nomura, Kazuko H. ; Dejima, Katsufumi ; Gengyo-Ando, Keiko ; Mitani, Shohei ; Sugahara, Kazuyuki ; Nomura, Kazuya. / Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans. In: Nature. 2003 ; Vol. 423, No. 6938. pp. 443-448.
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