Christmas-tree nanowire chips for dna separation

Sakon Rahong, Takao Yasui, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Annop Klamchuen, Meng Gang, He Yong, Fuwei Zhuge, Noritada Kaji, Yoshinobu Baba, Tomoji Kawai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We demonstrated the DNA separation by using the three dimensional Christmas-tree nanowire chips embedded in the microfluidic system to enhance the separation range and decrease the time consuming also. We synthesize the Christmas-tree nanowire by decorating Au catalyst along the nanowires structure by sputtering and growing the branch of nanowires by Vapor-Liquid-Solid (VLS) technique as a cycle. The DNA electrophoretic mobility in the Christmas- tree nanowire decreases when the pore size between nanowire decreases by growth time. The DNA migration in the Christmas-tree nanowire chip is faster than gel electrophoresis due to the rigidity of 3D nanowire structure. Consequently, we succeeded to separate the wide range of DNA molecules by mobility difference in 3D nanowire structure in short time.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages164-166
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Nanowires
DNA
Electrophoretic mobility
Electrophoresis
Microfluidics
Rigidity
Pore size
Sputtering
Gels
Vapors
Catalysts
Molecules
Liquids

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Rahong, S., Yasui, T., Yanagida, T., Kanai, M., Nagashima, K., Klamchuen, A., ... Kawai, T. (2013). Christmas-tree nanowire chips for dna separation. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 164-166). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.

Christmas-tree nanowire chips for dna separation. / Rahong, Sakon; Yasui, Takao; Yanagida, Takeshi; Kanai, Masaki; Nagashima, Kazuki; Klamchuen, Annop; Gang, Meng; Yong, He; Zhuge, Fuwei; Kaji, Noritada; Baba, Yoshinobu; Kawai, Tomoji.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 164-166 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rahong, S, Yasui, T, Yanagida, T, Kanai, M, Nagashima, K, Klamchuen, A, Gang, M, Yong, H, Zhuge, F, Kaji, N, Baba, Y & Kawai, T 2013, Christmas-tree nanowire chips for dna separation. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 1, Chemical and Biological Microsystems Society, pp. 164-166, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Rahong S, Yasui T, Yanagida T, Kanai M, Nagashima K, Klamchuen A et al. Christmas-tree nanowire chips for dna separation. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 164-166. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Rahong, Sakon ; Yasui, Takao ; Yanagida, Takeshi ; Kanai, Masaki ; Nagashima, Kazuki ; Klamchuen, Annop ; Gang, Meng ; Yong, He ; Zhuge, Fuwei ; Kaji, Noritada ; Baba, Yoshinobu ; Kawai, Tomoji. / Christmas-tree nanowire chips for dna separation. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 164-166 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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AU - Yasui, Takao

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AU - Kanai, Masaki

AU - Nagashima, Kazuki

AU - Klamchuen, Annop

AU - Gang, Meng

AU - Yong, He

AU - Zhuge, Fuwei

AU - Kaji, Noritada

AU - Baba, Yoshinobu

AU - Kawai, Tomoji

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AB - We demonstrated the DNA separation by using the three dimensional Christmas-tree nanowire chips embedded in the microfluidic system to enhance the separation range and decrease the time consuming also. We synthesize the Christmas-tree nanowire by decorating Au catalyst along the nanowires structure by sputtering and growing the branch of nanowires by Vapor-Liquid-Solid (VLS) technique as a cycle. The DNA electrophoretic mobility in the Christmas- tree nanowire decreases when the pore size between nanowire decreases by growth time. The DNA migration in the Christmas-tree nanowire chip is faster than gel electrophoresis due to the rigidity of 3D nanowire structure. Consequently, we succeeded to separate the wide range of DNA molecules by mobility difference in 3D nanowire structure in short time.

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