Chromatin-induced spindle assembly plays an important role inmetaphase congression of silkworm holocentric chromosomes

Hiroaki Mon, Man Lee, Kazuei Mita, Marian R. Goldsmith, Takahiro Kusakabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The kinetochore plays important roles in cell cycle progression. Interactions between chromosomes and spindle microtubules allow chromosomes to congress to the middle of the cell and to segregate the sister chromatids into daughter cells in mitosis. The chromosome passenger complex (CPC), composed of the Aurora B kinase and its regulatory subunits INCENP, Survivin, and Borealin, plays multiple roles in these chromosomal events. In the genome of the silkworm, Bombyx mori, which has holocentric chromosomes, the CPC components and their molecular interactions were highly conserved. In contrast to monocentric species, however, the silkworm CPC co-localized with the chromatin-driven spindles on the upper side of prometaphase chromosomes without forming bipolar mitotic spindles. Depletion of the CPC by RNAi arrested the cell cycle progression at prometaphase and disrupted the microtubule network of the chromatin-driven spindles. Interestingly, depletion of mitotic centromere-associated kinesin (MCAK) recovered formation of the microtubule network but did not overcome the cell cycle arrest at prometaphase. These results suggest that the CPC modulates the chromatin-induced spindle assembly and metaphase congression of silkworm holocentric chromosomes.

Original languageEnglish
Pages (from-to)40-50
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume45
Issue number1
DOIs
Publication statusPublished - Feb 1 2014

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Bombyx
Chromosomes
silkworms
Chromatin
chromatin
chromosomes
Prometaphase
Microtubules
microtubules
Cells
cell cycle
Cell Cycle
Aurora Kinase B
Kinetochores
Kinesin
kinesin
kinetochores
mitotic spindle apparatus
Spindle Apparatus
Chromatids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Chromatin-induced spindle assembly plays an important role inmetaphase congression of silkworm holocentric chromosomes. / Mon, Hiroaki; Lee, Man; Mita, Kazuei; Goldsmith, Marian R.; Kusakabe, Takahiro.

In: Insect Biochemistry and Molecular Biology, Vol. 45, No. 1, 01.02.2014, p. 40-50.

Research output: Contribution to journalArticle

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