Chromatographic resolution of glucosidic compounds, ginsenosides on polyethersulphone membrane, and its application to the quantitative immunoassay for ginseng saponins

Osamu Morinaga, Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A method has been devised for the chromatographic resolution of glucosidic compounds, ginseng saponins, on polyethersulphone (PES) membrane. The method results in good resolution and quantitative immunoassay for ginsenoside Rb1 (G-Rb1), G-Rc, and G-Rd in crude extracts of various ginsengs. The newly established method is simpler and applies for quantitative analysis. Ginsenosides developed by acetonitrile-water-acetic acid solvent system on a PES membrane were directly treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a ginsenoside-BSA conjugate on a PES membrane. Anti-G-Rb1 monoclonal antibody (MAb) was bound, and then a second antibody labeled with peroxidase directed against the first antibody. Finally a substrate reacted to the enzyme and gave staining. The stained membrane was scanned, and spots were analyzed quantitatively using NIH Image software. At least 62.5 ng of G-Rb1, G-Rc, and G-Rd were clearly detectable individually. Three ginsenosides can be analyzed quantitatively between 0.125 and 2.0 μg.

Original languageEnglish
Pages (from-to)1061-1066
Number of pages6
JournalGlycobiology
Volume15
Issue number10
DOIs
Publication statusPublished - Oct 1 2005

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Ginsenosides
Panax
Saponins
Immunoassay
Membranes
Bovine Serum Albumin
Antibodies
Complex Mixtures
Acetic Acid
Peroxidase
Software
Monoclonal Antibodies
Staining and Labeling
polyether sulfone
Water
Substrates
Enzymes
Chemical analysis
ginsenoside Rb1

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Chromatographic resolution of glucosidic compounds, ginsenosides on polyethersulphone membrane, and its application to the quantitative immunoassay for ginseng saponins. / Morinaga, Osamu; Fukuda, Noriko; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Glycobiology, Vol. 15, No. 10, 01.10.2005, p. 1061-1066.

Research output: Contribution to journalArticle

Morinaga, Osamu ; Fukuda, Noriko ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Chromatographic resolution of glucosidic compounds, ginsenosides on polyethersulphone membrane, and its application to the quantitative immunoassay for ginseng saponins. In: Glycobiology. 2005 ; Vol. 15, No. 10. pp. 1061-1066.
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