NP220s form a family of DNA-binding nuclear proteins originally found in human cell lines. Four isoforms of NP220 are produced in humans and mice probably by alternative splicing of two sequence units. They have RNA-recognition and arginine/serine rich motives commonly found in many nuclear proteins important for pre-mRNA processing. In order to analyze the function of NP220s, its gene was disrupted in chicken cell line DT40. For this, gemomic DNA clone of chicken NP220 was isolated and the gene was localized to chromosome 4q2.7-q2.8. Chicken NP220 conserves MH1 and zinc finger-like motives found in human and mouse NP220s. Despite its expression as a mRNA of 6 kb in wild type DT40 cells, disruption of the NP220 gene did not impair the growth of DT40 cells.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology