Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells

Yuichi Matsushima, Kiyoshi Matsumura, Shohji Ishii, Tomohiro Suzuki, Yoichi Matsuda, Yasuo Kitagawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

NP220s form a family of DNA-binding nuclear proteins originally found in human cell lines. Four isoforms of NP220 are produced in humans and mice probably by alternative splicing of two sequence units. They have RNA-recognition and arginine/serine rich motives commonly found in many nuclear proteins important for pre-mRNA processing. In order to analyze the function of NP220s, its gene was disrupted in chicken cell line DT40. For this, gemomic DNA clone of chicken NP220 was isolated and the gene was localized to chromosome 4q2.7-q2.8. Chicken NP220 conserves MH1 and zinc finger-like motives found in human and mouse NP220s. Despite its expression as a mRNA of 6 kb in wild type DT40 cells, disruption of the NP220 gene did not impair the growth of DT40 cells.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalCytotechnology
Volume33
Issue number1-3
DOIs
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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