Chromosomal organization, evolutionary relationship, and expression of zebrafish GnRH family members

Ming Wei Kuo, Show Wan Lou, John Postlethwait, Bon Chu Chung

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Multiple forms of gonadotropin-releasing hormone (GnRH) are found in different vertebrates. In this study, we have cloned cDNA encoding the full-length gnrh3 and gnrh2 from zebrafish brain and characterized their structure and expression patterns. We performed phylogenetic analysis and compared conserved syntenies in the region surrounding the GnRH genes from human, chicken, pufferfish, and zebrafish genores. The gnrh3 and gnrh2 genes were mapped to LG17 and LG21, respectively. The zebrafish genome appears to lack an ortholog to human GNRH1, and the human genome appears to lack an ortholog of gnrh3. Expression of gnrh3 began in the olfactory pit at 24-26 h postfertilization and expanded to the olfactory bulb during early larval stage. Expression of gnrh2 is always in the midbrain. In addition, GnRH is also expressed in boundary cells surrounding seminiferous cysts of the testis. Thus, this detailed phylogenetic, chromosomal comparison, and expression study defines the identity and the evolutionary relationship of two zebrafish gnrh genes. We propose a model describing the evolution of gnrh genes involving ancestral duplication of the genes followed by selective loss of one gene in some teleosts.

Original languageEnglish
Pages (from-to)629-639
Number of pages11
JournalJournal of Biomedical Science
Volume12
Issue number4
DOIs
Publication statusPublished - Jul 1 2005

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Zebrafish
Gonadotropin-Releasing Hormone
Genes
Organizations
Tetraodontiformes
Gene Duplication
Olfactory Bulb
Human Genome
Mesencephalon
Vertebrates
Testis
Cysts
Chickens
Complementary DNA
Genome
Brain

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Chromosomal organization, evolutionary relationship, and expression of zebrafish GnRH family members. / Kuo, Ming Wei; Lou, Show Wan; Postlethwait, John; Chung, Bon Chu.

In: Journal of Biomedical Science, Vol. 12, No. 4, 01.07.2005, p. 629-639.

Research output: Contribution to journalArticle

Kuo, Ming Wei ; Lou, Show Wan ; Postlethwait, John ; Chung, Bon Chu. / Chromosomal organization, evolutionary relationship, and expression of zebrafish GnRH family members. In: Journal of Biomedical Science. 2005 ; Vol. 12, No. 4. pp. 629-639.
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