Chromosome analysis after ex vivo expansion of CD34+ cells from human cord blood

Yoshio Katayama; Kanji Miyamoto; Katsuto Takenaka; Kenji Imajyo; Katsuji Shinagawa; Mine Harada

doi:10.1016/S0165-4608(00)00364-2

Chromosome analysis after ex vivo expansion of CD34⁺ cells from human cord blood

Yoshio Katayama, Kanji Miyamoto, Katsuto Takenaka, Kenji Imajyo, Katsuji Shinagawa, Mine Harada

Hematology, Oncology & Cardiovascular medicine

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Ex vivo expansion of cord blood hematopoietic progenitors is an attractive way to prepare a sufficient number of transplantable cells for cord blood transplantation in adult patients. The expanded cells need to have genetic stability. Karyotypic analysis of the expanded cells from cord blood CD34⁺ cells by 7-day culture with stem cell growth factor, interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor, and erythropoietin was performed. Several-fold increases in total cell number and CFU-GM were 186.7 ± 62.1 and 27.1 ± 9.4 (mean ± standard deviation of data from 6 samples), respectively. Karyotypes were analyzed in 600 expanded cells in total, and all of them showed normal karyotypes. This observation suggests that multifactor supported expansion of cord blood cells may not induce karyotypic abnormality, although a limited number of ex vivo expanded cells were tested.

Original language	English
Pages (from-to)	161-162
Number of pages	2
Journal	Cancer Genetics and Cytogenetics
Volume	125
Issue number	2
DOIs	https://doi.org/10.1016/S0165-4608(00)00364-2
Publication status	Published - Mar 1 2001

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0165-4608(00)00364-2

Cite this

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abstract = "Ex vivo expansion of cord blood hematopoietic progenitors is an attractive way to prepare a sufficient number of transplantable cells for cord blood transplantation in adult patients. The expanded cells need to have genetic stability. Karyotypic analysis of the expanded cells from cord blood CD34+ cells by 7-day culture with stem cell growth factor, interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor, and erythropoietin was performed. Several-fold increases in total cell number and CFU-GM were 186.7 ± 62.1 and 27.1 ± 9.4 (mean ± standard deviation of data from 6 samples), respectively. Karyotypes were analyzed in 600 expanded cells in total, and all of them showed normal karyotypes. This observation suggests that multifactor supported expansion of cord blood cells may not induce karyotypic abnormality, although a limited number of ex vivo expanded cells were tested.",

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AU - Katayama, Yoshio

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AU - Shinagawa, Katsuji

AU - Harada, Mine

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