Chronic stress, as well as acute stress, reduces BDNF mRNA expression in the rat hippocampus but less robustly

Shuji Murakami, Hiroki Imbe, Yoshihiro Morikawa, Chiharu Kubo, Emiko Senba

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

Daily restraint for 3 weeks was shown to atrophy dendrites of hippocampal pyramidal neurons in rats. Brain-derived neurotrophic factor (BDNF), which maintains neuronal survival and morphology, has been shown to decrease in response to acute stress. Plasma glucocorticoid (GC) and serotonergic projections from the raphe nuclei play major roles in reducing BDNF synthesis in the hippocampus. We investigated BDNF mRNA levels there, together with plasma GC levels, GC receptors in the hippocampus/hypothalamus and 5-HT synthesizing enzyme, tryptophan hydroxylase in the raphe nuclei, in animals chronically stressed for 1-3 weeks, using in situ hybridization and immunohistochemistry. In these animals, BDNF mRNA levels were significantly decreased in the hippocampus after 6 h of restraint, but the ability of restraint to reduce BDNF synthesis seemed less robust than that seen in acute stress models. HPA axis response to stress in these animals assessed by plasma GC levels was delayed and sustained, and the GC receptor in the paraventricular hypothalamic nucleus was increased at 1 week. Tryptophan hydroxylase immunoreactivity was increased in the median raphe nucleus at 2-3 weeks. Repetitive stress-induced reduction of BDNF may partly contribute to the neuronal atrophy/death and reduction of hippocampal volume observed both in animals and humans suffering chronic stress and/or depression.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalNeuroscience Research
Volume53
Issue number2
DOIs
Publication statusPublished - Oct 1 2005

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Brain-Derived Neurotrophic Factor
Hippocampus
Messenger RNA
Raphe Nuclei
Glucocorticoids
Tryptophan Hydroxylase
Glucocorticoid Receptors
Atrophy
Paraventricular Hypothalamic Nucleus
Pyramidal Cells
Dendrites
Hypothalamus
In Situ Hybridization
Serotonin
Immunohistochemistry
Enzymes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Chronic stress, as well as acute stress, reduces BDNF mRNA expression in the rat hippocampus but less robustly. / Murakami, Shuji; Imbe, Hiroki; Morikawa, Yoshihiro; Kubo, Chiharu; Senba, Emiko.

In: Neuroscience Research, Vol. 53, No. 2, 01.10.2005, p. 129-139.

Research output: Contribution to journalArticle

Murakami, Shuji ; Imbe, Hiroki ; Morikawa, Yoshihiro ; Kubo, Chiharu ; Senba, Emiko. / Chronic stress, as well as acute stress, reduces BDNF mRNA expression in the rat hippocampus but less robustly. In: Neuroscience Research. 2005 ; Vol. 53, No. 2. pp. 129-139.
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