Chronological change of distribution in nitric oxide and peptidergic neurons after rat small intestinal transplantation

T. Taguchi, R. Guo, K. Masumoto, O. Nada, M. Nakao, K. Yanai, S. Suita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background/Purpose: Nitric oxide (NO) has been considered one of the putative neurotransmitters of nonadrenergic, noncholinergic inhibitory neurons. To examine the effect of transplantation on NO neurons in the intestine, the distribution of NO neurons was examined and compared with that of peptide-containing neurons. Methods: A jejunal graft measuring about 20 cm was harvested from a Lewis rat, syngeneically transplanted as a Thiry-Vella loop, and later was replaced to the recipient small bowel 20 days after transplantation. Tissue specimens of the grafts were taken on days 1, 3, 6, 10, and 20, and 1 year after transplantation (n = 5 each). The distribution of the neurons was examined immunohistochemically, using antisera against protein gene product 9.5 (PGP 9.5, general neuronal marker), brain nitric oxide synthase (bNOS), vasoactive intestinal polypeptide (VIP), and substance R In addition, NADPH diaphorase staining also was performed to visualize NO neurons. Results: In the PGP 9.5 immunoreactivities, no significant difference in the distribution was observed among the controls and on any day after transplantation. However, the NADPHd activities markedly decreased in muscle layers, especially in the deep muscular layer on day 1 and 3, but quickly recovered by day 6. The distribution of bNOS immunoreactivities was almost same as that of NADPHd staining. The VIP and substance P immunoreactivities also decreased on day 1, and thereafter gradually recovered, and then became normal on day 20. Conclusions: Both the NO and peptidergic neurons markedly decreased just after transplantation, and the NO neurons recovered faster than the peptidergic neurons. These findings suggested that NO neurons might play an important role in the adaptation process of the graft in the early period after transplantation.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalJournal of Pediatric Surgery
Volume34
Issue number2
DOIs
Publication statusPublished - Feb 1999

Fingerprint

Nitric Oxide
Transplantation
Neurons
Vasoactive Intestinal Peptide
Transplants
Nitric Oxide Synthase
Staining and Labeling
NADPH Dehydrogenase
Brain
Substance P
Intestines
Neurotransmitter Agents
Immune Sera
Muscles
Peptides

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Chronological change of distribution in nitric oxide and peptidergic neurons after rat small intestinal transplantation. / Taguchi, T.; Guo, R.; Masumoto, K.; Nada, O.; Nakao, M.; Yanai, K.; Suita, S.

In: Journal of Pediatric Surgery, Vol. 34, No. 2, 02.1999, p. 341-345.

Research output: Contribution to journalArticle

Taguchi, T. ; Guo, R. ; Masumoto, K. ; Nada, O. ; Nakao, M. ; Yanai, K. ; Suita, S. / Chronological change of distribution in nitric oxide and peptidergic neurons after rat small intestinal transplantation. In: Journal of Pediatric Surgery. 1999 ; Vol. 34, No. 2. pp. 341-345.
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abstract = "Background/Purpose: Nitric oxide (NO) has been considered one of the putative neurotransmitters of nonadrenergic, noncholinergic inhibitory neurons. To examine the effect of transplantation on NO neurons in the intestine, the distribution of NO neurons was examined and compared with that of peptide-containing neurons. Methods: A jejunal graft measuring about 20 cm was harvested from a Lewis rat, syngeneically transplanted as a Thiry-Vella loop, and later was replaced to the recipient small bowel 20 days after transplantation. Tissue specimens of the grafts were taken on days 1, 3, 6, 10, and 20, and 1 year after transplantation (n = 5 each). The distribution of the neurons was examined immunohistochemically, using antisera against protein gene product 9.5 (PGP 9.5, general neuronal marker), brain nitric oxide synthase (bNOS), vasoactive intestinal polypeptide (VIP), and substance R In addition, NADPH diaphorase staining also was performed to visualize NO neurons. Results: In the PGP 9.5 immunoreactivities, no significant difference in the distribution was observed among the controls and on any day after transplantation. However, the NADPHd activities markedly decreased in muscle layers, especially in the deep muscular layer on day 1 and 3, but quickly recovered by day 6. The distribution of bNOS immunoreactivities was almost same as that of NADPHd staining. The VIP and substance P immunoreactivities also decreased on day 1, and thereafter gradually recovered, and then became normal on day 20. Conclusions: Both the NO and peptidergic neurons markedly decreased just after transplantation, and the NO neurons recovered faster than the peptidergic neurons. These findings suggested that NO neurons might play an important role in the adaptation process of the graft in the early period after transplantation.",
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AU - Yanai, K.

AU - Suita, S.

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AB - Background/Purpose: Nitric oxide (NO) has been considered one of the putative neurotransmitters of nonadrenergic, noncholinergic inhibitory neurons. To examine the effect of transplantation on NO neurons in the intestine, the distribution of NO neurons was examined and compared with that of peptide-containing neurons. Methods: A jejunal graft measuring about 20 cm was harvested from a Lewis rat, syngeneically transplanted as a Thiry-Vella loop, and later was replaced to the recipient small bowel 20 days after transplantation. Tissue specimens of the grafts were taken on days 1, 3, 6, 10, and 20, and 1 year after transplantation (n = 5 each). The distribution of the neurons was examined immunohistochemically, using antisera against protein gene product 9.5 (PGP 9.5, general neuronal marker), brain nitric oxide synthase (bNOS), vasoactive intestinal polypeptide (VIP), and substance R In addition, NADPH diaphorase staining also was performed to visualize NO neurons. Results: In the PGP 9.5 immunoreactivities, no significant difference in the distribution was observed among the controls and on any day after transplantation. However, the NADPHd activities markedly decreased in muscle layers, especially in the deep muscular layer on day 1 and 3, but quickly recovered by day 6. The distribution of bNOS immunoreactivities was almost same as that of NADPHd staining. The VIP and substance P immunoreactivities also decreased on day 1, and thereafter gradually recovered, and then became normal on day 20. Conclusions: Both the NO and peptidergic neurons markedly decreased just after transplantation, and the NO neurons recovered faster than the peptidergic neurons. These findings suggested that NO neurons might play an important role in the adaptation process of the graft in the early period after transplantation.

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