Class switching from γ3 to γ2b in a murine pre-B cell line

Tani Yoshihiko; Sugiyama Haruo; Maeda Toyoki; Miyake Seigou; Oka Yoshihiro; Ogawa Hiroyasu; Komori Toshihisa; Soma Toshihiro; Kishimoto Susumu

doi:10.1016/0161-5890(88)90060-0

Class switching from γ3 to γ2b in a murine pre-B cell line

Tani Yoshihiko, Sugiyama Haruo, Maeda Toyoki, Miyake Seigou, Oka Yoshihiro, Ogawa Hiroyasu, Komori Toshihisa, Soma Toshihiro, Kishimoto Susumu

Research output: Contribution to journal › Article › peer-review

Abstract

Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform V_H to DJ_H recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening C_H genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.

Original language	English
Pages (from-to)	127-136
Number of pages	10
Journal	Molecular Immunology
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/0161-5890(88)90060-0
Publication status	Published - Feb 1988
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology
Molecular Biology

Access to Document

10.1016/0161-5890(88)90060-0

Cite this

@article{38c2dfc9283245fc9194911ef3d7817d,

title = "Class switching from γ3 to γ2b in a murine pre-B cell line",

abstract = "Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.",

author = "Tani Yoshihiko and Sugiyama Haruo and Maeda Toyoki and Miyake Seigou and Oka Yoshihiro and Ogawa Hiroyasu and Komori Toshihisa and Soma Toshihiro and Kishimoto Susumu",

year = "1988",

month = feb,

doi = "10.1016/0161-5890(88)90060-0",

language = "English",

volume = "25",

pages = "127--136",

journal = "Immunochemistry",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "2",

}

TY - JOUR

T1 - Class switching from γ3 to γ2b in a murine pre-B cell line

AU - Yoshihiko, Tani

AU - Haruo, Sugiyama

AU - Toyoki, Maeda

AU - Seigou, Miyake

AU - Yoshihiro, Oka

AU - Hiroyasu, Ogawa

AU - Toshihisa, Komori

AU - Toshihiro, Soma

AU - Susumu, Kishimoto

PY - 1988/2

Y1 - 1988/2

N2 - Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.

AB - Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.

UR - http://www.scopus.com/inward/record.url?scp=0023720008&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023720008&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(88)90060-0

DO - 10.1016/0161-5890(88)90060-0

M3 - Article

C2 - 3131665

AN - SCOPUS:0023720008

VL - 25

SP - 127

EP - 136

JO - Immunochemistry

JF - Immunochemistry

SN - 0161-5890

IS - 2

ER -

Class switching from γ3 to γ2b in a murine pre-B cell line

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this