Abstract
Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.
| Original language | English |
|---|---|
| Pages (from-to) | 127-136 |
| Number of pages | 10 |
| Journal | Molecular Immunology |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1988 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Immunology
- Molecular Biology
Cite this
Class switching from γ3 to γ2b in a murine pre-B cell line. / Yoshihiko, Tani; Haruo, Sugiyama; Toyoki, Maeda; Seigou, Miyake; Yoshihiro, Oka; Hiroyasu, Ogawa; Toshihisa, Komori; Toshihiro, Soma; Susumu, Kishimoto.
In: Molecular Immunology, Vol. 25, No. 2, 02.1988, p. 127-136.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Class switching from γ3 to γ2b in a murine pre-B cell line
AU - Yoshihiko, Tani
AU - Haruo, Sugiyama
AU - Toyoki, Maeda
AU - Seigou, Miyake
AU - Yoshihiro, Oka
AU - Hiroyasu, Ogawa
AU - Toshihisa, Komori
AU - Toshihiro, Soma
AU - Susumu, Kishimoto
PY - 1988/2
Y1 - 1988/2
N2 - Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.
AB - Class switching from γ3 to γ2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from γ3 to γ2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from γ3 to γ2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.
UR - http://www.scopus.com/inward/record.url?scp=0023720008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023720008&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(88)90060-0
DO - 10.1016/0161-5890(88)90060-0
M3 - Article
C2 - 3131665
AN - SCOPUS:0023720008
VL - 25
SP - 127
EP - 136
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 2
ER -