Abstract
The heavy and light chain genes (μ and λ) of the human anti-adenocarcinoma monoclonal antibody HB4C5 (MAb-C5) were cloned from the human-human hybridoma cell line HB4C5 and co-expressed in Chinese hamster ovary (CHO) and COS-7 cells. ELISA assay and the RI imaging of the cancer tissue xenografted into nude mice showed that the recombinant MAb-C5 retained the original antigen binding activity. We then replaced the IgM constant region of this MAb-C5 heavy chain gene with the human IgG1 constant region gene and co-expressed it with the light chain gene. This recombination was confirmed by a complete DNA sequencing and Western-blot analysis. Despite the fact that the DNA sequence, the expressed protein size, and the assembly of heavy and light chains were indicated to be normal, the IgG1 type MAb-C5 could not bind to the original antigen. This result suggests that this antibody alters its antigen binding property upon class-switching.
| Original language | English |
|---|---|
| Pages (from-to) | 137-145 |
| Number of pages | 9 |
| Journal | Human Antibodies |
| Volume | 8 |
| Issue number | 3 |
| Publication status | Published - 1997 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
Cite this
Class-switching of the IgM type anti-adenocarcinoma human antibody HB4C5 into an IgG1 type resulted in the loss of the antigen binding ability. / Haruta, Hirotaka; Tachibana, Hirofumi; Mochizuki, Katsumi; Hashizume, Shuichi; Kusakabe, Kiyoko; Shirahata, Sanetaka; Murakami, Hiroki.
In: Human Antibodies, Vol. 8, No. 3, 1997, p. 137-145.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Class-switching of the IgM type anti-adenocarcinoma human antibody HB4C5 into an IgG1 type resulted in the loss of the antigen binding ability
AU - Haruta, Hirotaka
AU - Tachibana, Hirofumi
AU - Mochizuki, Katsumi
AU - Hashizume, Shuichi
AU - Kusakabe, Kiyoko
AU - Shirahata, Sanetaka
AU - Murakami, Hiroki
PY - 1997
Y1 - 1997
N2 - The heavy and light chain genes (μ and λ) of the human anti-adenocarcinoma monoclonal antibody HB4C5 (MAb-C5) were cloned from the human-human hybridoma cell line HB4C5 and co-expressed in Chinese hamster ovary (CHO) and COS-7 cells. ELISA assay and the RI imaging of the cancer tissue xenografted into nude mice showed that the recombinant MAb-C5 retained the original antigen binding activity. We then replaced the IgM constant region of this MAb-C5 heavy chain gene with the human IgG1 constant region gene and co-expressed it with the light chain gene. This recombination was confirmed by a complete DNA sequencing and Western-blot analysis. Despite the fact that the DNA sequence, the expressed protein size, and the assembly of heavy and light chains were indicated to be normal, the IgG1 type MAb-C5 could not bind to the original antigen. This result suggests that this antibody alters its antigen binding property upon class-switching.
AB - The heavy and light chain genes (μ and λ) of the human anti-adenocarcinoma monoclonal antibody HB4C5 (MAb-C5) were cloned from the human-human hybridoma cell line HB4C5 and co-expressed in Chinese hamster ovary (CHO) and COS-7 cells. ELISA assay and the RI imaging of the cancer tissue xenografted into nude mice showed that the recombinant MAb-C5 retained the original antigen binding activity. We then replaced the IgM constant region of this MAb-C5 heavy chain gene with the human IgG1 constant region gene and co-expressed it with the light chain gene. This recombination was confirmed by a complete DNA sequencing and Western-blot analysis. Despite the fact that the DNA sequence, the expressed protein size, and the assembly of heavy and light chains were indicated to be normal, the IgG1 type MAb-C5 could not bind to the original antigen. This result suggests that this antibody alters its antigen binding property upon class-switching.
UR - http://www.scopus.com/inward/record.url?scp=0030775628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030775628&partnerID=8YFLogxK
M3 - Article
C2 - 9322084
AN - SCOPUS:0030775628
VL - 8
SP - 137
EP - 145
JO - Human Antibodies
JF - Human Antibodies
SN - 1093-2607
IS - 3
ER -