Class-switching of the IgM type anti-adenocarcinoma human antibody HB4C5 into an IgG1 type resulted in the loss of the antigen binding ability

Hirotaka Haruta, Hirofumi Tachibana, Katsumi Mochizuki, Shuichi Hashizume, Kiyoko Kusakabe, Sanetaka Shirahata, Hiroki Murakami

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The heavy and light chain genes (μ and λ) of the human anti-adenocarcinoma monoclonal antibody HB4C5 (MAb-C5) were cloned from the human-human hybridoma cell line HB4C5 and co-expressed in Chinese hamster ovary (CHO) and COS-7 cells. ELISA assay and the RI imaging of the cancer tissue xenografted into nude mice showed that the recombinant MAb-C5 retained the original antigen binding activity. We then replaced the IgM constant region of this MAb-C5 heavy chain gene with the human IgG1 constant region gene and co-expressed it with the light chain gene. This recombination was confirmed by a complete DNA sequencing and Western-blot analysis. Despite the fact that the DNA sequence, the expressed protein size, and the assembly of heavy and light chains were indicated to be normal, the IgG1 type MAb-C5 could not bind to the original antigen. This result suggests that this antibody alters its antigen binding property upon class-switching.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalHuman Antibodies
Volume8
Issue number3
DOIs
Publication statusPublished - 1997

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Class-switching of the IgM type anti-adenocarcinoma human antibody HB4C5 into an IgG1 type resulted in the loss of the antigen binding ability'. Together they form a unique fingerprint.

Cite this