TY - JOUR
T1 - Classification of the binding modes in bovine serum albumin using terminally substituted alkane analogues
AU - Takehara, Kô
AU - Morinaga, Yuki
AU - Nakashima, Shinya
AU - Matsuoka, Shiro
AU - Kamaya, Hiroshi
AU - Ueda, Issaku
PY - 2006/12
Y1 - 2006/12
N2 - With the fluorescence probe of 8-anilino-1-naphthalenesulfonate (ANS), the binding modes of terminally substituted alkane analogues (CnX; X = COOH, OH, CHO, NH3, CONH2) to bovine serum albumin (BSA) were investigated using a competitive binding technique. The Scatchard plot of the fluorometric titration of BSA with ANS showed that the maximum binding number of ANS, nmax, was 3.81, with the binding constant, K bnd, of 1.42 × 106 mol-1 dm3. The binding modes of CnX to BSA were analyzed based on the fluorometric titration of the ANS and BSA mixture with CnX. C nCOOH completely displaced the ANS bound to BSA, whereas C nOH and CnCHO displaced only about 40% of the ANS bound to BSA. In contrast, CnNH2 and CnCONH2 displaced very little bound ANS. By comparing these results, we classified the binding modes of CnX to BSA into three types. Two of them are detectable with the ANS fluorescence and the remaining one is not detectable with the fluorescence. 2006
AB - With the fluorescence probe of 8-anilino-1-naphthalenesulfonate (ANS), the binding modes of terminally substituted alkane analogues (CnX; X = COOH, OH, CHO, NH3, CONH2) to bovine serum albumin (BSA) were investigated using a competitive binding technique. The Scatchard plot of the fluorometric titration of BSA with ANS showed that the maximum binding number of ANS, nmax, was 3.81, with the binding constant, K bnd, of 1.42 × 106 mol-1 dm3. The binding modes of CnX to BSA were analyzed based on the fluorometric titration of the ANS and BSA mixture with CnX. C nCOOH completely displaced the ANS bound to BSA, whereas C nOH and CnCHO displaced only about 40% of the ANS bound to BSA. In contrast, CnNH2 and CnCONH2 displaced very little bound ANS. By comparing these results, we classified the binding modes of CnX to BSA into three types. Two of them are detectable with the ANS fluorescence and the remaining one is not detectable with the fluorescence. 2006
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U2 - 10.2116/analsci.22.1571
DO - 10.2116/analsci.22.1571
M3 - Article
C2 - 17159317
AN - SCOPUS:36148996222
SN - 0910-6340
VL - 22
SP - 1571
EP - 1575
JO - Analytical Sciences
JF - Analytical Sciences
IS - 12
ER -