Cleft palate formation after palatal fusion occurs due to the rupture of epithelial basement membranes

Chisato Sakuma, Hideto Imura, Tomohiro Yamada, Toshio Sugahara, Azumi Hirata, Yayoi Ikeda, Nagato Natsume

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate and hydronephrosis in the mouse embryo. Cleft palate occurs due to failure in palatal grow, but the underlying mechanisms are unclear. We investigated the mechanisms of cleft palate development in TCDD-exposed mouse embryos. We administered olive oil (control group) or TCDD diluted in olive oil (40 μg/kg) via gastric tubes to pregnant mice on gestational day (GD) 12. Embryos of control and TCDD-exposed groups were removed from pregnant mice on GD 14 and GD 15, respectively. One mouse embryo from the control group had anteroposterior palatal fusion. Palatal fusion was observed in three TCDD-exposed mouse embryos. Palates of TCDD-exposed mice fused from the interior to the middle of the palates, while the palates were separated in the posterior region. The middle of the embryonic palatal shelves in TCDD-exposed animals was narrow and split at the fusional position. At this position, palatal and blood cells were dispersed from the palatal tissue and the epithelium was split, with a discontinuous basement membrane. The results suggest that decreased intercellular adhesion or insufficient tissue strength of the palatal shelves may be involved in the development of cleft palate following palatal fusion.

Original languageEnglish
Pages (from-to)2027-2031
Number of pages5
JournalJournal of Cranio-Maxillofacial Surgery
Volume46
Issue number12
DOIs
Publication statusPublished - Dec 2018

Fingerprint

Cleft Palate
Basement Membrane
Rupture
Embryonic Structures
Palate
Control Groups
Hydronephrosis
Polychlorinated Dibenzodioxins
Blood Cells
Stomach
Epithelium

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

Cleft palate formation after palatal fusion occurs due to the rupture of epithelial basement membranes. / Sakuma, Chisato; Imura, Hideto; Yamada, Tomohiro; Sugahara, Toshio; Hirata, Azumi; Ikeda, Yayoi; Natsume, Nagato.

In: Journal of Cranio-Maxillofacial Surgery, Vol. 46, No. 12, 12.2018, p. 2027-2031.

Research output: Contribution to journalArticle

Sakuma, Chisato ; Imura, Hideto ; Yamada, Tomohiro ; Sugahara, Toshio ; Hirata, Azumi ; Ikeda, Yayoi ; Natsume, Nagato. / Cleft palate formation after palatal fusion occurs due to the rupture of epithelial basement membranes. In: Journal of Cranio-Maxillofacial Surgery. 2018 ; Vol. 46, No. 12. pp. 2027-2031.
@article{0557c0eeaccd4ffab93846d69c734dbb,
title = "Cleft palate formation after palatal fusion occurs due to the rupture of epithelial basement membranes",
abstract = "2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate and hydronephrosis in the mouse embryo. Cleft palate occurs due to failure in palatal grow, but the underlying mechanisms are unclear. We investigated the mechanisms of cleft palate development in TCDD-exposed mouse embryos. We administered olive oil (control group) or TCDD diluted in olive oil (40 μg/kg) via gastric tubes to pregnant mice on gestational day (GD) 12. Embryos of control and TCDD-exposed groups were removed from pregnant mice on GD 14 and GD 15, respectively. One mouse embryo from the control group had anteroposterior palatal fusion. Palatal fusion was observed in three TCDD-exposed mouse embryos. Palates of TCDD-exposed mice fused from the interior to the middle of the palates, while the palates were separated in the posterior region. The middle of the embryonic palatal shelves in TCDD-exposed animals was narrow and split at the fusional position. At this position, palatal and blood cells were dispersed from the palatal tissue and the epithelium was split, with a discontinuous basement membrane. The results suggest that decreased intercellular adhesion or insufficient tissue strength of the palatal shelves may be involved in the development of cleft palate following palatal fusion.",
author = "Chisato Sakuma and Hideto Imura and Tomohiro Yamada and Toshio Sugahara and Azumi Hirata and Yayoi Ikeda and Nagato Natsume",
year = "2018",
month = "12",
doi = "10.1016/j.jcms.2018.09.016",
language = "English",
volume = "46",
pages = "2027--2031",
journal = "Journal of Cranio-Maxillo-Facial Surgery",
issn = "1010-5182",
publisher = "Churchill Livingstone",
number = "12",

}

TY - JOUR

T1 - Cleft palate formation after palatal fusion occurs due to the rupture of epithelial basement membranes

AU - Sakuma, Chisato

AU - Imura, Hideto

AU - Yamada, Tomohiro

AU - Sugahara, Toshio

AU - Hirata, Azumi

AU - Ikeda, Yayoi

AU - Natsume, Nagato

PY - 2018/12

Y1 - 2018/12

N2 - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate and hydronephrosis in the mouse embryo. Cleft palate occurs due to failure in palatal grow, but the underlying mechanisms are unclear. We investigated the mechanisms of cleft palate development in TCDD-exposed mouse embryos. We administered olive oil (control group) or TCDD diluted in olive oil (40 μg/kg) via gastric tubes to pregnant mice on gestational day (GD) 12. Embryos of control and TCDD-exposed groups were removed from pregnant mice on GD 14 and GD 15, respectively. One mouse embryo from the control group had anteroposterior palatal fusion. Palatal fusion was observed in three TCDD-exposed mouse embryos. Palates of TCDD-exposed mice fused from the interior to the middle of the palates, while the palates were separated in the posterior region. The middle of the embryonic palatal shelves in TCDD-exposed animals was narrow and split at the fusional position. At this position, palatal and blood cells were dispersed from the palatal tissue and the epithelium was split, with a discontinuous basement membrane. The results suggest that decreased intercellular adhesion or insufficient tissue strength of the palatal shelves may be involved in the development of cleft palate following palatal fusion.

AB - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate and hydronephrosis in the mouse embryo. Cleft palate occurs due to failure in palatal grow, but the underlying mechanisms are unclear. We investigated the mechanisms of cleft palate development in TCDD-exposed mouse embryos. We administered olive oil (control group) or TCDD diluted in olive oil (40 μg/kg) via gastric tubes to pregnant mice on gestational day (GD) 12. Embryos of control and TCDD-exposed groups were removed from pregnant mice on GD 14 and GD 15, respectively. One mouse embryo from the control group had anteroposterior palatal fusion. Palatal fusion was observed in three TCDD-exposed mouse embryos. Palates of TCDD-exposed mice fused from the interior to the middle of the palates, while the palates were separated in the posterior region. The middle of the embryonic palatal shelves in TCDD-exposed animals was narrow and split at the fusional position. At this position, palatal and blood cells were dispersed from the palatal tissue and the epithelium was split, with a discontinuous basement membrane. The results suggest that decreased intercellular adhesion or insufficient tissue strength of the palatal shelves may be involved in the development of cleft palate following palatal fusion.

UR - http://www.scopus.com/inward/record.url?scp=85054638976&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054638976&partnerID=8YFLogxK

U2 - 10.1016/j.jcms.2018.09.016

DO - 10.1016/j.jcms.2018.09.016

M3 - Article

C2 - 30322778

AN - SCOPUS:85054638976

VL - 46

SP - 2027

EP - 2031

JO - Journal of Cranio-Maxillo-Facial Surgery

JF - Journal of Cranio-Maxillo-Facial Surgery

SN - 1010-5182

IS - 12

ER -