"Click-made" biaryl-linker improving efficiency in protein labelling for the membrane target protein of a bioactive compound

Yoko Nakamura, Sho Inomata, Makoto Ebine, Yoshiyuki Manabe, Izumi Iwakura, Minoru Ueda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We report on the design, synthesis and assessment of a novel biaryl-linked (BArL) molecular probe for the exploration of low-abundant target proteins for bioactive compounds based on the activity based protein profiling (ABPP) approach. Surprisingly, the performance of the BArL probe was better than that of the stepwise tagging approach that is considered to be the most effective method used in ABPP study.

Original languageEnglish
Pages (from-to)83-85
Number of pages3
JournalOrganic and Biomolecular Chemistry
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

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activity (biology)
Labeling
marking
Membrane Proteins
membranes
proteins
Membranes
Molecular Probes
Proteins
probes
synthesis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

"Click-made" biaryl-linker improving efficiency in protein labelling for the membrane target protein of a bioactive compound. / Nakamura, Yoko; Inomata, Sho; Ebine, Makoto; Manabe, Yoshiyuki; Iwakura, Izumi; Ueda, Minoru.

In: Organic and Biomolecular Chemistry, Vol. 9, No. 1, 01.01.2011, p. 83-85.

Research output: Contribution to journalArticle

Nakamura, Yoko ; Inomata, Sho ; Ebine, Makoto ; Manabe, Yoshiyuki ; Iwakura, Izumi ; Ueda, Minoru. / "Click-made" biaryl-linker improving efficiency in protein labelling for the membrane target protein of a bioactive compound. In: Organic and Biomolecular Chemistry. 2011 ; Vol. 9, No. 1. pp. 83-85.
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