Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR

Ryota Sozaki, Tatsuro Tajiri, Mayumi Higashi, Yoshiaki Kinoshita, Sakura Tanaka, Kenichi Kouhashi, Masazumi Tsuneyoshi, Tomoaki Taguchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Introduction: Recently, determining the MYCN status in neuroblastoma (NB) using the quantitative PCR (Q-PCR) and FISH instead of the Southern blotting (SB) has been recommended. In order to assess the implications of the gene dosage of MYCN in NB, the MYCN status was evaluated using Q-PCR on DNA extracted from small areas of NB specimens obtained using laser capture microdissection (LCM). Materials and methods: MYCN gene dosages (MYCN/NAGK) were determined in 63 primary NB block samples, as well as in 243 microdissected tissues from 63 samples using Q-PCR. In 23 of 63 cases, the MYCN gene status was evaluated using FISH. Results: Nine block samples with the amplification of MYCN based on SB showed a remarkable increase of the MYCN gene dosage using Q-PCR. Twelve of 54 block samples with no amplification of MYCN based on SB showed a slight increase of the MYCN gene dosage (3.56 ≧ MYCN/NAGK > 1.84), and 8 of these 12 cases were in the advanced stage. Among these 12 cases, 1 case had several LCM areas with a high copy number of MYCN and several LCM areas which showed no increase of MYCN gene. Another case showed a slight increase in the MYCN gene dosage (3.65 ≦ MYCN/NAGK ≦ 4.82) in all LCM areas. In addition, a large number of cells with the MYCN gain were found using FISH in the block sample. In 2 other cases of 12 cases, although no LCM areas showed an increased gene dosage of MYCN, a small number of cells with MYCN amplification were found using FISH were found in the block sample. Conclusion: A slight increase in the gene dosage of MYCN detected by Q-PCR may indicate that the NB tissue contains a small number of cells with the MYCN amplification or a large number of cells with the MYCN gain, which are associated with the aggressive progression of NB.

Original languageEnglish
Pages (from-to)1095-1100
Number of pages6
JournalPediatric surgery international
Volume24
Issue number10
DOIs
Publication statusPublished - Oct 1 2008

Fingerprint

Gene Dosage
Neuroblastoma
Laser Capture Microdissection
Polymerase Chain Reaction
Cell Count
Southern Blotting
Genes
DNA

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR. / Sozaki, Ryota; Tajiri, Tatsuro; Higashi, Mayumi; Kinoshita, Yoshiaki; Tanaka, Sakura; Kouhashi, Kenichi; Tsuneyoshi, Masazumi; Taguchi, Tomoaki.

In: Pediatric surgery international, Vol. 24, No. 10, 01.10.2008, p. 1095-1100.

Research output: Contribution to journalArticle

Sozaki, Ryota ; Tajiri, Tatsuro ; Higashi, Mayumi ; Kinoshita, Yoshiaki ; Tanaka, Sakura ; Kouhashi, Kenichi ; Tsuneyoshi, Masazumi ; Taguchi, Tomoaki. / Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR. In: Pediatric surgery international. 2008 ; Vol. 24, No. 10. pp. 1095-1100.
@article{0b4bce3378694c729c14acb89ce67eee,
title = "Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR",
abstract = "Introduction: Recently, determining the MYCN status in neuroblastoma (NB) using the quantitative PCR (Q-PCR) and FISH instead of the Southern blotting (SB) has been recommended. In order to assess the implications of the gene dosage of MYCN in NB, the MYCN status was evaluated using Q-PCR on DNA extracted from small areas of NB specimens obtained using laser capture microdissection (LCM). Materials and methods: MYCN gene dosages (MYCN/NAGK) were determined in 63 primary NB block samples, as well as in 243 microdissected tissues from 63 samples using Q-PCR. In 23 of 63 cases, the MYCN gene status was evaluated using FISH. Results: Nine block samples with the amplification of MYCN based on SB showed a remarkable increase of the MYCN gene dosage using Q-PCR. Twelve of 54 block samples with no amplification of MYCN based on SB showed a slight increase of the MYCN gene dosage (3.56 ≧ MYCN/NAGK > 1.84), and 8 of these 12 cases were in the advanced stage. Among these 12 cases, 1 case had several LCM areas with a high copy number of MYCN and several LCM areas which showed no increase of MYCN gene. Another case showed a slight increase in the MYCN gene dosage (3.65 ≦ MYCN/NAGK ≦ 4.82) in all LCM areas. In addition, a large number of cells with the MYCN gain were found using FISH in the block sample. In 2 other cases of 12 cases, although no LCM areas showed an increased gene dosage of MYCN, a small number of cells with MYCN amplification were found using FISH were found in the block sample. Conclusion: A slight increase in the gene dosage of MYCN detected by Q-PCR may indicate that the NB tissue contains a small number of cells with the MYCN amplification or a large number of cells with the MYCN gain, which are associated with the aggressive progression of NB.",
author = "Ryota Sozaki and Tatsuro Tajiri and Mayumi Higashi and Yoshiaki Kinoshita and Sakura Tanaka and Kenichi Kouhashi and Masazumi Tsuneyoshi and Tomoaki Taguchi",
year = "2008",
month = "10",
day = "1",
doi = "10.1007/s00383-008-2228-3",
language = "English",
volume = "24",
pages = "1095--1100",
journal = "Pediatric Surgery International",
issn = "0179-0358",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR

AU - Sozaki, Ryota

AU - Tajiri, Tatsuro

AU - Higashi, Mayumi

AU - Kinoshita, Yoshiaki

AU - Tanaka, Sakura

AU - Kouhashi, Kenichi

AU - Tsuneyoshi, Masazumi

AU - Taguchi, Tomoaki

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Introduction: Recently, determining the MYCN status in neuroblastoma (NB) using the quantitative PCR (Q-PCR) and FISH instead of the Southern blotting (SB) has been recommended. In order to assess the implications of the gene dosage of MYCN in NB, the MYCN status was evaluated using Q-PCR on DNA extracted from small areas of NB specimens obtained using laser capture microdissection (LCM). Materials and methods: MYCN gene dosages (MYCN/NAGK) were determined in 63 primary NB block samples, as well as in 243 microdissected tissues from 63 samples using Q-PCR. In 23 of 63 cases, the MYCN gene status was evaluated using FISH. Results: Nine block samples with the amplification of MYCN based on SB showed a remarkable increase of the MYCN gene dosage using Q-PCR. Twelve of 54 block samples with no amplification of MYCN based on SB showed a slight increase of the MYCN gene dosage (3.56 ≧ MYCN/NAGK > 1.84), and 8 of these 12 cases were in the advanced stage. Among these 12 cases, 1 case had several LCM areas with a high copy number of MYCN and several LCM areas which showed no increase of MYCN gene. Another case showed a slight increase in the MYCN gene dosage (3.65 ≦ MYCN/NAGK ≦ 4.82) in all LCM areas. In addition, a large number of cells with the MYCN gain were found using FISH in the block sample. In 2 other cases of 12 cases, although no LCM areas showed an increased gene dosage of MYCN, a small number of cells with MYCN amplification were found using FISH were found in the block sample. Conclusion: A slight increase in the gene dosage of MYCN detected by Q-PCR may indicate that the NB tissue contains a small number of cells with the MYCN amplification or a large number of cells with the MYCN gain, which are associated with the aggressive progression of NB.

AB - Introduction: Recently, determining the MYCN status in neuroblastoma (NB) using the quantitative PCR (Q-PCR) and FISH instead of the Southern blotting (SB) has been recommended. In order to assess the implications of the gene dosage of MYCN in NB, the MYCN status was evaluated using Q-PCR on DNA extracted from small areas of NB specimens obtained using laser capture microdissection (LCM). Materials and methods: MYCN gene dosages (MYCN/NAGK) were determined in 63 primary NB block samples, as well as in 243 microdissected tissues from 63 samples using Q-PCR. In 23 of 63 cases, the MYCN gene status was evaluated using FISH. Results: Nine block samples with the amplification of MYCN based on SB showed a remarkable increase of the MYCN gene dosage using Q-PCR. Twelve of 54 block samples with no amplification of MYCN based on SB showed a slight increase of the MYCN gene dosage (3.56 ≧ MYCN/NAGK > 1.84), and 8 of these 12 cases were in the advanced stage. Among these 12 cases, 1 case had several LCM areas with a high copy number of MYCN and several LCM areas which showed no increase of MYCN gene. Another case showed a slight increase in the MYCN gene dosage (3.65 ≦ MYCN/NAGK ≦ 4.82) in all LCM areas. In addition, a large number of cells with the MYCN gain were found using FISH in the block sample. In 2 other cases of 12 cases, although no LCM areas showed an increased gene dosage of MYCN, a small number of cells with MYCN amplification were found using FISH were found in the block sample. Conclusion: A slight increase in the gene dosage of MYCN detected by Q-PCR may indicate that the NB tissue contains a small number of cells with the MYCN amplification or a large number of cells with the MYCN gain, which are associated with the aggressive progression of NB.

UR - http://www.scopus.com/inward/record.url?scp=52449110522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52449110522&partnerID=8YFLogxK

U2 - 10.1007/s00383-008-2228-3

DO - 10.1007/s00383-008-2228-3

M3 - Article

C2 - 18726105

AN - SCOPUS:52449110522

VL - 24

SP - 1095

EP - 1100

JO - Pediatric Surgery International

JF - Pediatric Surgery International

SN - 0179-0358

IS - 10

ER -