Clinical implications of serum anti-p53 antibodies for patients with gastric carcinoma

Yoshihiko Maehara, Yoshihiro Kakeji, Akihiro Watanabe, Hideo Baba, Hiroki Kusumoto, Shunji Kohnoe, Keizo Sugimachi

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Abstract

BACKGROUND. Mutations of p53 can lead to the production of anti-p53 antibodies in sera of cancer patients. Before this study, the value of preoperative serum anti-p53 antibodies in determining the prognoses of patients with gastric carcinoma had yet to be determined. METHODS. The authors used a highly specific enzyme-linked immunosorbent assay (ELISA) kit (Pharma Cell, France) to determine the preoperative presence of serum anti- p53 antibodies in 120 patients with gastric carcinoma. The relation between the positivity of serum anti-p53 antibodies and p53 abnormal staining of gastric carcinoma tissues was examined. Clinicopathologic characteristics and prognoses of these patients were given attention. RESULTS. Anti-p53 antibodies were detected in 19.2%(23 of 120) of these patients with gastric carcinoma. Among those who were positive for anti-p53 antibodies, female patients were predominant, the depth of invasion was greater, and liver metastasis was present, as compared with those who were negative for anti- p53 antibodies. Regarding other factors, there were no differences between those who were positive or negative for anti-p53 antibodies. Gastric carcinoma tissues had a 60.9%(14 of 23) positivity rate of p53 staining with and-p53 antibodies and a 33.0%(32 of 97) negativity rate, and this difference was statistically significant (P < 0.05). The survival time of patients with anti-p53 antibodies in their sera was shorter than that of subjects with sera negative for anti-p53 antibodies (P < 0.05). The presence of anti-p53 antibodies was not an independent prognostic factor in multivariate analysis. CONCLUSIONS. Serum assay of anti-p53 antibodies is a rapid and readily facilitated test for predicting tumor advancement, depth of invasion, and liver metastasis, and it will show a poorer prognosis for surgically treated patients with gastric carci-noma.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalCancer
Volume85
Issue number2
DOIs
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Maehara, Y., Kakeji, Y., Watanabe, A., Baba, H., Kusumoto, H., Kohnoe, S., & Sugimachi, K. (1999). Clinical implications of serum anti-p53 antibodies for patients with gastric carcinoma. Cancer, 85(2), 302-308. https://doi.org/10.1002/(SICI)1097-0142(19990115)85:2<302::AID-CNCR6>3.0.CO;2-6