Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma

Tsuyoshi Etoh, Hiroaki Ueo, Hiroshi Inoue, Hiroshi Sato, Tohru Utsunomiya, Graham F. Barnard, Seigo Kitano, Masaki Mori

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Abstract

Background: Recurrent and metastatic carcinoma of the colorectum remains a major problem. This may be ascribed to the presence of micrometastasis at diagnosis. The purpose of this study was to analyze prospectively the clinical value of detecting K-ras mutations in the perioperative circulating blood from patients with colorectal carcinoma. Methods: Twenty-four patients whose tumor carried mutations in codon 12 of the K-ras gene were studied for the presence of cancer cells in perioperative blood samples, in particular, tumor drainage samples. A detection assay using CD45 immunomagnetic separation plus nested mutant allele specific amplification (MASA) was performed. Results: K-ras mutations in CD45 negative cells in tumor drainage blood were detected in 7 (29.2%) of 24 patients. There was no significant relationship between the presence of a K-ras mutation and clinicopathological features. Four (57.1%) of the seven patients with a positive K-ras mutation in drainage blood had early recurrent disease. Of the 17 patients with no K-ras mutation, none developed metastatic disease. The recurrence rate of the K-ras mutation positive group was higher than that of the K-ras mutation negative group (P < 01). There was a significant difference, regarding prognosis, between K-ras mutation positive and negative groups (P < .01). Conclusions: This preliminary study demonstrates that the detection of circulating cancer cells in the tumor drainage blood by our new assay system may provide a predictor of recurrence and metastasis of colorectal cancer.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalAnnals of Surgical Oncology
Volume8
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

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Drainage
Colorectal Neoplasms
Mutation
Neoplasms
Immunomagnetic Separation
Recurrence
Neoplasm Micrometastasis
ras Genes
Codon
Alleles
Neoplasm Metastasis
Carcinoma

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

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Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma. / Etoh, Tsuyoshi; Ueo, Hiroaki; Inoue, Hiroshi; Sato, Hiroshi; Utsunomiya, Tohru; Barnard, Graham F.; Kitano, Seigo; Mori, Masaki.

In: Annals of Surgical Oncology, Vol. 8, No. 5, 01.01.2001, p. 407-412.

Research output: Contribution to journalArticle

Etoh, Tsuyoshi ; Ueo, Hiroaki ; Inoue, Hiroshi ; Sato, Hiroshi ; Utsunomiya, Tohru ; Barnard, Graham F. ; Kitano, Seigo ; Mori, Masaki. / Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma. In: Annals of Surgical Oncology. 2001 ; Vol. 8, No. 5. pp. 407-412.
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abstract = "Background: Recurrent and metastatic carcinoma of the colorectum remains a major problem. This may be ascribed to the presence of micrometastasis at diagnosis. The purpose of this study was to analyze prospectively the clinical value of detecting K-ras mutations in the perioperative circulating blood from patients with colorectal carcinoma. Methods: Twenty-four patients whose tumor carried mutations in codon 12 of the K-ras gene were studied for the presence of cancer cells in perioperative blood samples, in particular, tumor drainage samples. A detection assay using CD45 immunomagnetic separation plus nested mutant allele specific amplification (MASA) was performed. Results: K-ras mutations in CD45 negative cells in tumor drainage blood were detected in 7 (29.2{\%}) of 24 patients. There was no significant relationship between the presence of a K-ras mutation and clinicopathological features. Four (57.1{\%}) of the seven patients with a positive K-ras mutation in drainage blood had early recurrent disease. Of the 17 patients with no K-ras mutation, none developed metastatic disease. The recurrence rate of the K-ras mutation positive group was higher than that of the K-ras mutation negative group (P < 01). There was a significant difference, regarding prognosis, between K-ras mutation positive and negative groups (P < .01). Conclusions: This preliminary study demonstrates that the detection of circulating cancer cells in the tumor drainage blood by our new assay system may provide a predictor of recurrence and metastasis of colorectal cancer.",
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