Clinical significance of low expression of Prostasin mRNA in human gastric cancer

Katsuya Sakashita, Koshi Mimori, Fumiaki Tanaka, Kouichirou Tahara, Hiroshi Inoue, Tetsuji Sawada, Masaichi Ohira, Kosei Hirakawa, Masaki Mori

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Background: Prostasin is considered to have suppressive activities against tumor progression. The aim of this study was to clarify its clinical significance in gastric cancer. Methods: Tumor and the corresponding normal samples were prepared from a total of 108 gastric cancer patients. Prostasin expression was assayed by real time reverse transcription (RT)-polymerase chain reaction (PCR) and by immunohistochemistry. Epigenetic silencing of the expression was examined by demethylation treatment. Survival was examined in Kaplan-Meier plots, and the relationship between its expression and clinicopathologic factors was statistically analyzed. Results: Prostasin mRNA expression was significantly down-regulated in tumor tissues. Immunohistochemistry confirmed loss of Prostasin expression in gastric cancer. Gastric cancer cell lines that did not express Prostasin mRNA retrieved its expression following demethylation treatment. In those patients whose tumor expressed Prostasin mRNA at reduced levels, we observed shorter survival (P=0.0110), due to a higher incidence of lymph node metastasis (P=0.0087), lymphatic permeation (P=0.0542) and venous permeation (P=0.0492). Conclusions: Prostasin mRNA expression was frequently down-regulated in gastric cancer. Loss of expression might be regulated by epigenetic factors, contributing to the shorter survival. Reduced Prostasin mRNA expression might be a novel indicator for biological aggressiveness in gastric cancer.

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalJournal of Surgical Oncology
Volume98
Issue number7
DOIs
Publication statusPublished - Dec 1 2008

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

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