The expression of pyrimidine nucleoside phosphorylase (PyNPase) mRNA in tumor (T) and normal (N) biopsy specimens obtained from 41 cases of colorectal carcinoma was examined by the reverse transcriptase-polymerase chain reaction (RT-PCR). The higher T/N ratio of the expression of PyNPase mRNA correlated significantly with the presence of lymph vessel invasion (p = 0.039), the positive lymph node metastasis (p = 0.014) and the advanced stage of the disease (p = 0.014). There was a significant correlation between the results determined by enzyme activity and those determined by RT-PCR (p = 0.005). The findings suggested that the determination of PyNPase mRNA by RT-PCR may give useful information on tumor aggressiveness of colorectal carcinoma and this method can be used instead of enzyme activity.
|Number of pages||4|
|Journal||International journal of oncology|
|Publication status||Published - Mar 1 1997|
All Science Journal Classification (ASJC) codes
- Cancer Research