Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11

Isao Abe, Kanji Okumoto, Shigehiko Tamura, Yukio Fujiki

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-termius. Pex11p comigrated in SDS-PAGE with a 28-kDA peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.

Original languageEnglish
Pages (from-to)468-472
Number of pages5
JournalFEBS Letters
Volume431
Issue number3
DOIs
Publication statusPublished - Jul 24 1998

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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