Clonal deletion of self-Mls-reactive thymocytes at the early stage in H-2-compatible but Mls-disparate radiation chimeras

M. Ogimoto, Y. Yoshikai, G. Matsuzaki, S. Ohga, K. Matsumoto, K. Nomoto

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Abstract

Clonal deletion of T cells capable of recognizing both host-type Mls and donor-type Mls occurred in the peripheral mature T-cell pool in radiation bone marrow chimeras of two H-2-compatible Mls-disparate strain combinations of AKR/J(H-2(k),Thy-1.1,Mls-1a) and C3H/He(H-2(k),Thy-1.2-Mls-1b). In order to determine further the stage at which the clonal deletion occurs in thymus, we examined the kinetics of thymocytes bearing Vβ6 capable of recognizing Mls-1a in both C3H/He → AKR/J and AKR/J → C3H/He chimeras. An almost complete replacement from host-derived cells to donor-derived cells occurred by Day 21 after reconstitution in both chimeras. At this stage, CD4+CD8+ double-positive thymocytes contained an appreciable number of cells that expressed Vβ6 on their surface, albeit at low intensity, whereas CD4 or CD8 single-positive thymocytes which expressed a high density of Vβ6 were virtually abolished in both C3H → AKR and AKR → C3H chimeras on Day 21. These results suggest that clonal deletion of self-Mls-reactive T cells begins at an early stage when the thymocytes interact with the early appearing donor-derived haematopoietic cells and relatively radio-resistant host-derived cells in thymus of radiation bone marrow chimeras.

Original languageEnglish
Pages (from-to)482-486
Number of pages5
JournalImmunology
Volume69
Issue number3
Publication statusPublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Ogimoto, M., Yoshikai, Y., Matsuzaki, G., Ohga, S., Matsumoto, K., & Nomoto, K. (1990). Clonal deletion of self-Mls-reactive thymocytes at the early stage in H-2-compatible but Mls-disparate radiation chimeras. Immunology, 69(3), 482-486.