Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting

Atsushi Suzuki, Y. w. Zheng, K. Fukao, H. Nakauchi, H. Taniguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Although hepatic stem cells are believed to exist and play a critical role in developing and regenerating liver, little is known about their cell surface specificity or differentiation capabilities. To make prospective studies of hepatic stem cells possible, we established an in vitro culture system for identification and characterization of hepatic stem/progenitor cells. By combining this culture system with fluorescence activated cell sorting (FACS), a population of cells that were capable of forming large colonies and providing their descendants for relative longer period was isolated from fetal mouse livers. These data suggest that hepatic stem/progenitor cells with high proliferative potential are existent in the developing mouse liver, and that they are enriched by using flow cytometry.

Original languageEnglish
Pages (from-to)393-396
Number of pages4
JournalCell Transplantation
Volume10
Issue number4-5
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Stem cells
Sorting
Hepatocytes
Stem Cells
Cells
Liver
Cell culture
Flow cytometry
Flow Cytometry
Fluorescence
Prospective Studies
Population

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Transplantation

Cite this

Suzuki, A., Zheng, Y. W., Fukao, K., Nakauchi, H., & Taniguchi, H. (2001). Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting. Cell Transplantation, 10(4-5), 393-396.

Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting. / Suzuki, Atsushi; Zheng, Y. w.; Fukao, K.; Nakauchi, H.; Taniguchi, H.

In: Cell Transplantation, Vol. 10, No. 4-5, 2001, p. 393-396.

Research output: Contribution to journalArticle

Suzuki, A, Zheng, YW, Fukao, K, Nakauchi, H & Taniguchi, H 2001, 'Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting', Cell Transplantation, vol. 10, no. 4-5, pp. 393-396.
Suzuki, Atsushi ; Zheng, Y. w. ; Fukao, K. ; Nakauchi, H. ; Taniguchi, H. / Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting. In: Cell Transplantation. 2001 ; Vol. 10, No. 4-5. pp. 393-396.
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