Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver

Atsushi Suzuki, Yun Wen Zheng, Shin Kaneko, Masafumi Onodera, Katashi Fukao, Hiromitsu Nakauchi, Hideki Taniguchi

Research output: Contribution to journalArticle

310 Citations (Scopus)

Abstract

Using flow cytometry and single cell-based assays, we prospectively identified hepatic stem cells with multi-lineage differentiation potential and self-renewing capability. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When cells that expanded in vitro were transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. These data indicate that self-renewing pluripotent stem cells persist in the developing mouse liver and that such cells can be induced to become cells of other organs of endodermal origin under appropriate microenvironment. Manipulation of hepatic stem cells may provide new insight into therapies for diseases of the digestive system.

Original languageEnglish
Pages (from-to)173-184
Number of pages12
JournalJournal of Cell Biology
Volume156
Issue number1
DOIs
Publication statusPublished - Jan 7 2002

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Pluripotent Stem Cells
Hepatocytes
Liver
Stem Cells
Digestive System Diseases
Acinar Cells
Bile Ducts
Pancreas
Flow Cytometry
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver. / Suzuki, Atsushi; Zheng, Yun Wen; Kaneko, Shin; Onodera, Masafumi; Fukao, Katashi; Nakauchi, Hiromitsu; Taniguchi, Hideki.

In: Journal of Cell Biology, Vol. 156, No. 1, 07.01.2002, p. 173-184.

Research output: Contribution to journalArticle

Suzuki, Atsushi ; Zheng, Yun Wen ; Kaneko, Shin ; Onodera, Masafumi ; Fukao, Katashi ; Nakauchi, Hiromitsu ; Taniguchi, Hideki. / Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver. In: Journal of Cell Biology. 2002 ; Vol. 156, No. 1. pp. 173-184.
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