Abstract
Background: In Japan, chronic active Epstein-Barr virus infection (CAEBV) may manifest with infection of T-cells or NK-cells, clonal lymphoid proliferations, and overt lymphoid malignancy. These EBV-positive lymphoproliferative disorders (EBV +LPD) of childhood are related to, but distinct from the infectious mononucleosis-like CAEBV seen in Western populations. The clonal nature of viral infection within lymphoid subsets of patients with EBV +LPD of childhood is not well described. Objectives: Viral distribution and clonotype were assessed within T-cell subsets, NK-cells, and CD34 +stem cells following high purity cell sorting. Study design: Six Japanese patients with EBV +LPD of childhood (3 T-cell LPD and 3 NK-cell LPD) were recruited. Prior to immunochemotherapy, viral loads and clonal analyses of T-cell subsets, NK-cells, and CD34 +stem cells were studied by high-accuracy cell sorting (>99.5%), Southern blotting and real-time polymerase chain reaction. Results: Patient 1 had a monoclonal proliferation of EBV-infected γδT-cells and carried a lower copy number of EBV in αβT-cells. Patients 2 and 3 had clonal expansions of EBV-infected CD4 +T-cells, and lower EBV load in NK-cells. Patients 4, 5 and 6 had EBV +NK-cell expansions with higher EBV load than T-cells. EBV-terminal repeats were determined as clonal bands in the minor targeted populations of 5 patients. The size of terminal repeats indicated the same clonotype in minor subsets as in the major subsets of four patients. EBV was not, however, detected in the bone marrow-derived CD34 +stem cells of patients. Conclusions: A single EBV clonotype may infect multiple NK-cell and T-cell subsets of patients with EBV +LPD of childhood. CD34 +stem cells are spared, suggesting infection of more differentiated elements.
| Original language | English |
|---|---|
| Pages (from-to) | 31-37 |
| Number of pages | 7 |
| Journal | Journal of Clinical Virology |
| Volume | 51 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 1 2011 |
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All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
Cite this
Clonal origin of Epstein-Barr virus (EBV)-infected T/NK-cell subpopulations in EBV-positive T/NK-cell lymphoproliferative disorders of childhood. / Ohga, Shouichi; Ishimura, Masataka; Yoshimoto, Goichi; Miyamoto, Toshihiro; Takada, Hidetoshi; Tanaka, Tamami; Ohshima, Koichi; Ogawa, Yoshiyasu; Imadome, Ken Ichi; Abe, Yasunobu; Akashi, Koichi; Hara, Toshiro.
In: Journal of Clinical Virology, Vol. 51, No. 1, 01.05.2011, p. 31-37.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clonal origin of Epstein-Barr virus (EBV)-infected T/NK-cell subpopulations in EBV-positive T/NK-cell lymphoproliferative disorders of childhood
AU - Ohga, Shouichi
AU - Ishimura, Masataka
AU - Yoshimoto, Goichi
AU - Miyamoto, Toshihiro
AU - Takada, Hidetoshi
AU - Tanaka, Tamami
AU - Ohshima, Koichi
AU - Ogawa, Yoshiyasu
AU - Imadome, Ken Ichi
AU - Abe, Yasunobu
AU - Akashi, Koichi
AU - Hara, Toshiro
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Background: In Japan, chronic active Epstein-Barr virus infection (CAEBV) may manifest with infection of T-cells or NK-cells, clonal lymphoid proliferations, and overt lymphoid malignancy. These EBV-positive lymphoproliferative disorders (EBV +LPD) of childhood are related to, but distinct from the infectious mononucleosis-like CAEBV seen in Western populations. The clonal nature of viral infection within lymphoid subsets of patients with EBV +LPD of childhood is not well described. Objectives: Viral distribution and clonotype were assessed within T-cell subsets, NK-cells, and CD34 +stem cells following high purity cell sorting. Study design: Six Japanese patients with EBV +LPD of childhood (3 T-cell LPD and 3 NK-cell LPD) were recruited. Prior to immunochemotherapy, viral loads and clonal analyses of T-cell subsets, NK-cells, and CD34 +stem cells were studied by high-accuracy cell sorting (>99.5%), Southern blotting and real-time polymerase chain reaction. Results: Patient 1 had a monoclonal proliferation of EBV-infected γδT-cells and carried a lower copy number of EBV in αβT-cells. Patients 2 and 3 had clonal expansions of EBV-infected CD4 +T-cells, and lower EBV load in NK-cells. Patients 4, 5 and 6 had EBV +NK-cell expansions with higher EBV load than T-cells. EBV-terminal repeats were determined as clonal bands in the minor targeted populations of 5 patients. The size of terminal repeats indicated the same clonotype in minor subsets as in the major subsets of four patients. EBV was not, however, detected in the bone marrow-derived CD34 +stem cells of patients. Conclusions: A single EBV clonotype may infect multiple NK-cell and T-cell subsets of patients with EBV +LPD of childhood. CD34 +stem cells are spared, suggesting infection of more differentiated elements.
AB - Background: In Japan, chronic active Epstein-Barr virus infection (CAEBV) may manifest with infection of T-cells or NK-cells, clonal lymphoid proliferations, and overt lymphoid malignancy. These EBV-positive lymphoproliferative disorders (EBV +LPD) of childhood are related to, but distinct from the infectious mononucleosis-like CAEBV seen in Western populations. The clonal nature of viral infection within lymphoid subsets of patients with EBV +LPD of childhood is not well described. Objectives: Viral distribution and clonotype were assessed within T-cell subsets, NK-cells, and CD34 +stem cells following high purity cell sorting. Study design: Six Japanese patients with EBV +LPD of childhood (3 T-cell LPD and 3 NK-cell LPD) were recruited. Prior to immunochemotherapy, viral loads and clonal analyses of T-cell subsets, NK-cells, and CD34 +stem cells were studied by high-accuracy cell sorting (>99.5%), Southern blotting and real-time polymerase chain reaction. Results: Patient 1 had a monoclonal proliferation of EBV-infected γδT-cells and carried a lower copy number of EBV in αβT-cells. Patients 2 and 3 had clonal expansions of EBV-infected CD4 +T-cells, and lower EBV load in NK-cells. Patients 4, 5 and 6 had EBV +NK-cell expansions with higher EBV load than T-cells. EBV-terminal repeats were determined as clonal bands in the minor targeted populations of 5 patients. The size of terminal repeats indicated the same clonotype in minor subsets as in the major subsets of four patients. EBV was not, however, detected in the bone marrow-derived CD34 +stem cells of patients. Conclusions: A single EBV clonotype may infect multiple NK-cell and T-cell subsets of patients with EBV +LPD of childhood. CD34 +stem cells are spared, suggesting infection of more differentiated elements.
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UR - http://www.scopus.com/inward/citedby.url?scp=79953748328&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2011.01.014
DO - 10.1016/j.jcv.2011.01.014
M3 - Article
C2 - 21377409
AN - SCOPUS:79953748328
VL - 51
SP - 31
EP - 37
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 1
ER -