Cloning and characterization of a gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus

Hatsumi Taniguchi, Shigeo Kubomura, Hideaki Hirano, Kumiko Mizue, Midori Ogawa, Yasuo Mizuguchi

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20 Citations (Scopus)

Abstract

A new thermostable hemolsin (σ-VPH) gene was cloned from a Kanagawa-negative Vibrio parahaemolyticus strain into vector pBR322 in Escherichia coli K12. The nucleotide and amino acid sequences had np homology with those of the thermostable direct hemolysin (TDH) which causes the Kanagawa phenomenon, and of the thermolabile hemolysin (TLH) of V. parahaemolyticus. The gene was present in all V. parahaemolyticus strains tested and also in one strain of V. damsela.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalFEMS microbiology letters
Volume67
Issue number3
DOIs
Publication statusPublished - Feb 1990
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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