Cloning and characterization of a ribonuclease L inhibitor from the silkworm, Bombyx mori

Takuji Maeda, Man Lee, Yoshitaka Miyagawa, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The ribonuclease L (RNase L) pathway plays an important role in the response of cells to double-stranded RNA (dsRNA) during the events such as virus infection. Ribonuclease L inhibitor (RLI) belonging to the ABC transporter family is known as a regulator of the RNase L pathway. The homologs of RLI were reported in many organisms including the fruit fly and mosquito, but their functions in insects and arthropods have not been elucidated to date. In the present study, we cloned a cDNA of a silkworm RLI homolog, termed BmRLI, and its nucleotide sequence was determined. RT-PCR analysis revealed that the expression of BmRLI mRNA was marked in the testis, ovary and fat body. From the cDNA, recombinant protein with an apparent molecular mass of 69 kDa was expressed in Escherichia coli and cultured insect cells. Although no obvious effect of up-regulation of the BmRLI expression on RNAi was observed, its down-regulation slightly reduced RNAi efficiency.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume16
Issue number1
DOIs
Publication statusPublished - Feb 1 2005

Fingerprint

Bombyx
Cloning
Ribonucleases
Organism Cloning
RNA Interference
Insects
Complementary DNA
Fat Body
ATP-Binding Cassette Transporters
Double-Stranded RNA
Arthropods
Molecular mass
Virus Diseases
Fruits
Culicidae
Viruses
Recombinant Proteins
Diptera
Escherichia coli
Testis

All Science Journal Classification (ASJC) codes

  • Genetics
  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Cloning and characterization of a ribonuclease L inhibitor from the silkworm, Bombyx mori. / Maeda, Takuji; Lee, Man; Miyagawa, Yoshitaka; Koga, Katsumi; Kawaguchi, Yutaka; Kusakabe, Takahiro.

In: DNA Sequence - Journal of DNA Sequencing and Mapping, Vol. 16, No. 1, 01.02.2005, p. 21-27.

Research output: Contribution to journalArticle

Maeda, Takuji ; Lee, Man ; Miyagawa, Yoshitaka ; Koga, Katsumi ; Kawaguchi, Yutaka ; Kusakabe, Takahiro. / Cloning and characterization of a ribonuclease L inhibitor from the silkworm, Bombyx mori. In: DNA Sequence - Journal of DNA Sequencing and Mapping. 2005 ; Vol. 16, No. 1. pp. 21-27.
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