Cloning and developmental expression of the murine homolog of doublecortin

Naoki Matsuo, Shoko Kawamoto, Kenichi Matsubara, Kousaku Okubo

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23 Citations (Scopus)

Abstract

While analyzing active genes in neonatal mouse hippocampus by quantitative 3'-cDNA collection, we identified a highly conserved murine homolog of doublecortin, the causative gene of X-linked lissencephaly (XLIS) and subcortical laminar heterotopia (SCLH) syndrome. The m-doublecortin cDNA contains nearly 8 kb 3' UTR homologous to hs-doublecortin. and it was mapped to the X chromosome. The expression of m-doublecortin is limited to the developing CNS, especially the cortical plate, supporting that XLIS/SCLH syndrome is associated with an arrest of neuronal migration in the cerebral cortex. The m-doublecortin mRNA was absent in the ventricular zone where neuronal precursors proliferate, and interestingly it was found in various brain structures that are not typically affected in patients with this syndrome.

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number3
DOIs
Publication statusPublished - Nov 27 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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