Cloning and expression of an α-2,8-polysialyltransferase (STX) from Xenopus laevis

Mariko Kudo, Eiji Takayama, Kosuke Tashiro, Hiroshi Fukamachi, Takahiro Nakata, Takushi Tadakuma, Ken Kitajima, Yasuo Inoue, Koichiro Shiokawa

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Polysialic acid (polySia) is a carbohydrate structure found on neural cell adhesion molecules (N-CAM). Two polysialyltransferases (polySiaTs) that catalyze synthesis of polySia have been described, and designated PST-1/PST/ST8SiaIV and STX/ST8SiaII. We cloned a polySiaT (xSTX) from a nonmammalian vertebrate, Xenopus laevis. xSTX had 80% amino acid similarity to the rat STX. This clone induced polySia expression when transfected into polySia-negative COS-1 cells. Northern blot analysis of whole embryos at different stages of development revealed that xSTX mRNA was most abundantly expressed in premetamorphic stages. The relative level of xSTX and N-CAM mRNAs was also examined and found to change in parallel to the extent of polysialylation on N-CAM. In adult tissues, the expression of xSTX mRNA was restricted to brain, eye and heart, which also expressed polySia. These results suggest that xSTX is the major enzyme responsible for the synthesis of polysialylated N-CAM in embryos at certain stages of development and also in adult tissues.

Original languageEnglish
Pages (from-to)771-777
Number of pages7
JournalGlycobiology
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 1 1998

Fingerprint

Cloning
Xenopus laevis
Neural Cell Adhesion Molecules
Organism Cloning
Messenger RNA
Embryonic Structures
Tissue
COS Cells
Northern Blotting
Vertebrates
Rats
Brain
Clone Cells
Carbohydrates
CMP-N-acetylneuraminate-poly-alpha-2,8-sialosyl sialyltransferase
polysialic acid
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Kudo, M., Takayama, E., Tashiro, K., Fukamachi, H., Nakata, T., Tadakuma, T., ... Shiokawa, K. (1998). Cloning and expression of an α-2,8-polysialyltransferase (STX) from Xenopus laevis. Glycobiology, 8(8), 771-777. https://doi.org/10.1093/glycob/8.8.771

Cloning and expression of an α-2,8-polysialyltransferase (STX) from Xenopus laevis. / Kudo, Mariko; Takayama, Eiji; Tashiro, Kosuke; Fukamachi, Hiroshi; Nakata, Takahiro; Tadakuma, Takushi; Kitajima, Ken; Inoue, Yasuo; Shiokawa, Koichiro.

In: Glycobiology, Vol. 8, No. 8, 01.08.1998, p. 771-777.

Research output: Contribution to journalArticle

Kudo, M, Takayama, E, Tashiro, K, Fukamachi, H, Nakata, T, Tadakuma, T, Kitajima, K, Inoue, Y & Shiokawa, K 1998, 'Cloning and expression of an α-2,8-polysialyltransferase (STX) from Xenopus laevis', Glycobiology, vol. 8, no. 8, pp. 771-777. https://doi.org/10.1093/glycob/8.8.771
Kudo, Mariko ; Takayama, Eiji ; Tashiro, Kosuke ; Fukamachi, Hiroshi ; Nakata, Takahiro ; Tadakuma, Takushi ; Kitajima, Ken ; Inoue, Yasuo ; Shiokawa, Koichiro. / Cloning and expression of an α-2,8-polysialyltransferase (STX) from Xenopus laevis. In: Glycobiology. 1998 ; Vol. 8, No. 8. pp. 771-777.
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