Cloning and expression of cDNA for rat O6-methylguanine-DNA methyltransferse

Sakumi Kunihiko, Akiko Shiraishi, Hiroshi Hayakawa, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

cDNA for O6-methylguanine-DNA methyltransferase was isolated by screening rat liver cDNA libraries, using as a probe the human cDNA sequence for methyltransferase. The rat cDNA encodes a protein with 209 amino acid residues. The predicted amino acid sequence of the rat methyltransferase exhibits considerable homology with those of the human, yeast and bacterial enzymes, especially around putative methyl acceptor sites. When the cDNA was placed under control of the lac promoter and expressed in methyltransferase-deficient Escherichia coli (ada-, ogt-) cells, a characteristic methyltransferase protein was produced. The rat DNA methyltransferase thus expressed could complement the biological defects of the E.coli cell caused by lack of its own DNA methyltransferases; e.g. increased sensitivity to alkylating agents in terms of both cell death and mutation induction

Original languageEnglish
Pages (from-to)5597-5601
Number of pages5
JournalNucleic acids research
Volume19
Issue number20
DOIs
Publication statusPublished - Oct 25 1991

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Cloning
Methyltransferases
CDNA
Rats
Organism Cloning
DNA
Complementary DNA
Escherichia coli
Amino acids
Escherichia Coli
Proteins
Cell
Protein
Cell death
Protein Methyltransferases
Liver
Yeast
Amino Acids
Amino Acid Sequence
Screening

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Cloning and expression of cDNA for rat O6-methylguanine-DNA methyltransferse. / Kunihiko, Sakumi; Shiraishi, Akiko; Hayakawa, Hiroshi; Sekiguchi, Mutsuo.

In: Nucleic acids research, Vol. 19, No. 20, 25.10.1991, p. 5597-5601.

Research output: Contribution to journalArticle

Kunihiko, Sakumi ; Shiraishi, Akiko ; Hayakawa, Hiroshi ; Sekiguchi, Mutsuo. / Cloning and expression of cDNA for rat O6-methylguanine-DNA methyltransferse. In: Nucleic acids research. 1991 ; Vol. 19, No. 20. pp. 5597-5601.
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