Cloning and expression of human endothelin-1 receptor cDNA

Kiminori Hosoda, Kazuwa Nakao, Hiroshi-Arai, Shin ichi Suga, Yoshihiro Ogawa, Masashi Mukoyama, Gotaro Shirakami, Yoshihiko Saito, Shigetada Nakanishi, Hiroo Imura

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 ≥ ET-2 ≫ ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 × 10-7 M ET-1 under voltage clamp at -60 mV, while a much weaker response was produced by 1 × 10-7 M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalFEBS Letters
Volume287
Issue number1-2
DOIs
Publication statusPublished - Aug 5 1991
Externally publishedYes

Fingerprint

Endothelin A Receptors
Cloning
Endothelin-1
Organism Cloning
Complementary DNA
Tissue
Clamping devices
Blood vessels
Gene Library
RNA
Amino Acids
COS Cells
Electric potential
Xenopus
Northern Blotting
Placenta
Oocytes
Blood Vessels
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Hosoda, K., Nakao, K., Hiroshi-Arai, Suga, S. I., Ogawa, Y., Mukoyama, M., ... Imura, H. (1991). Cloning and expression of human endothelin-1 receptor cDNA. FEBS Letters, 287(1-2), 23-26. https://doi.org/10.1016/0014-5793(91)80007-P

Cloning and expression of human endothelin-1 receptor cDNA. / Hosoda, Kiminori; Nakao, Kazuwa; Hiroshi-Arai; Suga, Shin ichi; Ogawa, Yoshihiro; Mukoyama, Masashi; Shirakami, Gotaro; Saito, Yoshihiko; Nakanishi, Shigetada; Imura, Hiroo.

In: FEBS Letters, Vol. 287, No. 1-2, 05.08.1991, p. 23-26.

Research output: Contribution to journalArticle

Hosoda, K, Nakao, K, Hiroshi-Arai, Suga, SI, Ogawa, Y, Mukoyama, M, Shirakami, G, Saito, Y, Nakanishi, S & Imura, H 1991, 'Cloning and expression of human endothelin-1 receptor cDNA', FEBS Letters, vol. 287, no. 1-2, pp. 23-26. https://doi.org/10.1016/0014-5793(91)80007-P
Hosoda K, Nakao K, Hiroshi-Arai, Suga SI, Ogawa Y, Mukoyama M et al. Cloning and expression of human endothelin-1 receptor cDNA. FEBS Letters. 1991 Aug 5;287(1-2):23-26. https://doi.org/10.1016/0014-5793(91)80007-P
Hosoda, Kiminori ; Nakao, Kazuwa ; Hiroshi-Arai ; Suga, Shin ichi ; Ogawa, Yoshihiro ; Mukoyama, Masashi ; Shirakami, Gotaro ; Saito, Yoshihiko ; Nakanishi, Shigetada ; Imura, Hiroo. / Cloning and expression of human endothelin-1 receptor cDNA. In: FEBS Letters. 1991 ; Vol. 287, No. 1-2. pp. 23-26.
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