Cloning and expression of two carbonyl reductase-like 20β-hydroxysteroid dehydrogenase cDNAs in ovarian follicles of rainbow trout (Oncorhynchus mykiss)

Guijun Guan, Minoru Tanaka, Takashi Todo, Graham Young, Michiyasu Yoshikuni, Yoshitaka Nagahama

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In salmonid fish, 20β-hydroxysteroid dehydrogenase (20β-HSD) is a key enzyme involved in the production of oocyte maturation-inducing hormone (MIH), 17α,20β-dihydroxy-4-pregnen-3-one. Here we report the isolation of two cDNAs which encode proteins with high homology to carbonyl reductase-like 20β-HSD (CR/20β-HSD) from rainbow trout (Oncorhynchus mykiss) ovarian follicles. Genomic DNA analysis showed that the two CR/20β-HSD cDNAs are derived from two different genes. Northern blot and RT PCR analysis demonstrated that trout CR/20β-HSDs are broadly expressed in various tissues. Enzymatic characterization using recombinant CR/20β-HSD proteins produced in E. coli showed that the product of one of the two cDNAs had both 20β-HSD and CR activity, but the other had neither activity. Although the functional significance of the two genes remains unresolved, these results clearly demonstrate the presence of two distinct CR/20β-HSD transcripts in the trout ovary.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number1
DOIs
Publication statusPublished - Feb 5 1999
Externally publishedYes

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20-Hydroxysteroid Dehydrogenases
Alcohol Oxidoreductases
Ovarian Follicle
Cloning
Oncorhynchus mykiss
Organism Cloning
Complementary DNA
Trout
Genes
Northern Blotting
Fish
Escherichia coli
Oocytes
Ovary
Fishes
Proteins
Hormones
Tissue
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and expression of two carbonyl reductase-like 20β-hydroxysteroid dehydrogenase cDNAs in ovarian follicles of rainbow trout (Oncorhynchus mykiss). / Guan, Guijun; Tanaka, Minoru; Todo, Takashi; Young, Graham; Yoshikuni, Michiyasu; Nagahama, Yoshitaka.

In: Biochemical and Biophysical Research Communications, Vol. 255, No. 1, 05.02.1999, p. 123-128.

Research output: Contribution to journalArticle

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AU - Tanaka, Minoru

AU - Todo, Takashi

AU - Young, Graham

AU - Yoshikuni, Michiyasu

AU - Nagahama, Yoshitaka

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