Cloning and expression of two carbonyl reductase-like 20β-hydroxysteroid dehydrogenase cDNAs in ovarian follicles of rainbow trout (Oncorhynchus mykiss)

Guijun Guan, Minoru Tanaka, Takashi Todo, Graham Young, Michiyasu Yoshikuni, Yoshitaka Nagahama

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

In salmonid fish, 20β-hydroxysteroid dehydrogenase (20β-HSD) is a key enzyme involved in the production of oocyte maturation-inducing hormone (MIH), 17α,20β-dihydroxy-4-pregnen-3-one. Here we report the isolation of two cDNAs which encode proteins with high homology to carbonyl reductase-like 20β-HSD (CR/20β-HSD) from rainbow trout (Oncorhynchus mykiss) ovarian follicles. Genomic DNA analysis showed that the two CR/20β-HSD cDNAs are derived from two different genes. Northern blot and RT PCR analysis demonstrated that trout CR/20β-HSDs are broadly expressed in various tissues. Enzymatic characterization using recombinant CR/20β-HSD proteins produced in E. coli showed that the product of one of the two cDNAs had both 20β-HSD and CR activity, but the other had neither activity. Although the functional significance of the two genes remains unresolved, these results clearly demonstrate the presence of two distinct CR/20β-HSD transcripts in the trout ovary.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number1
DOIs
Publication statusPublished - Feb 5 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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